Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

Immunoprecipitation:Sphingosine Kinase 1/SPHK1 AntibodyNBP3-29344] -Detection of human SPHK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SPHK1 antibody NBP3-29344 used for IP at 10 µg/mg lysate. SPHK1 was also immunoprecipitated by rabbit anti-SPHK1., which recognizes a downstream epitope. For blotting immunoprecipitated SPHK1, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Note: The more slowly migrating bands are believed to represent additional forms of SPHK1 owing to recognition by multiple antibodies each of which was directed toward distinctly different epitopes on SPHK1.

• Reactivity: Hu
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Sphingosine Kinase 1/SPHK1 Antibody - BSA Free Summary

• Immunogen: between 1 and 50
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPHK1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate buffer, pH 7 to 8
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

• EC 2.7.1.91
• SK1
• sphingosine kinase 1
• SPHK1
• SPHKSK 1
• SPK 1
• SPK
• SPK1

Background

Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPHK1