Sphingosine Kinase 1/SPHK1 [p Ser225] Antibody

Western Blot: Sphingosine Kinase 1/SPHK1 [p Ser225] Antibody [NBP3-23421] - Western blot image of recombinant his-tagged human Sphingosine Kinase 1/SPHK1 protein that was phosphorylated with ERK-2. Blots were probed with anti-Sphingosine Kinase 1/SPHK1 (Central Region) (NBP3-23415; lanes 1-3) and anti-Sphingosine Kinase 1/SPHK1 (Ser-225) (NBP3-23421; lanes 4-6). Both antibodies were used in the presence of no peptide (lanes 1 & 4), phospho-Sphingosine Kinase 1/SPHK1 (Ser-225) peptide (lanes 2 & 5), or unphosphorylated Sphingosine Kinase 1/SPHK1 (Ser-225) peptide (lanes 3 & 6).

Western Blot: Sphingosine Kinase 1/SPHK1 [p Ser225] Antibody [NBP3-23421] - Western blot of HeLa stimulated with calyculin A (lanes 1-4). The blots were untreated (lane 1 & 3) or treated with lambda phosphatase (lane 2 & 4), then probed with anti-Sphingosine Kinase 1/SPHK1 (Central region) NBP3-23415 (lanes 1 & 2) or anti-Sphingosine Kinase 1/SPHK1 (Ser-225) NBP3-23421 (lanes 3 & 4).

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Sphingosine Kinase 1/SPHK1 [p Ser225] Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Phospho-Sphingosine Kinase 1/SPHK1 (Ser-225) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding serine 225 in human Sphingosine Kinase 1/SPHK1. This sequence has 4 amino acid differences from mouse and 5 from rat Sphingosine Kinase 1/SPHK1, and is not homologous to sequences in SK2. The conserved site in rat and mouse Sphingosine Kinase 1/SPHK1 is at serine 224. [UniProt# Q9NYA1]
• Modification: p Ser225
• Specificity: This antibody was cross-adsorbed to non-specific phosphoserine and unphosphorylated Sphingosine Kinase 1/SPHK1 (Ser-225) peptide before affinity purification using phospho-Sphingosine Kinase 1/SPHK1 (Ser-225) peptide. The purified antibody detects a 47 kDa full-length recombinant human Sphingosine Kinase 1/SPHK1 protein phosphorylated with ERK2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPHK1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for Sphingosine Kinase 1/SPHK1 [p Ser225] Antibody

• EC 2.7.1.91
• SK1
• sphingosine kinase 1
• SPHK1
• SPHKSK 1
• SPK 1
• SPK
• SPK1

Background

Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPHK1