Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody [NBP2-20472] - A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SPHK1 protein stained by SPHK1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP2-20472] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Sphingosine Kinase 1/SPHK1 antibody (NBP2-20472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Sphingosine Kinase 1/SPHK1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Sphingosine Kinase 1/SPHK1. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPHK1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

• EC 2.7.1.91
• SK1
• sphingosine kinase 1
• SPHK1
• SPHKSK 1
• SPK 1
• SPK
• SPK1

Background

Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Sphingosine Kinase 1/SPHK1 Antibody (NBP2-20472)(1)

Publication using NBP2-20472

• Bhadwal P, Randhawa V, Vaiphei K et al. Clinical relevance of CERK and SPHK1 in breast cancer and their association with metastasis and drug resistance Scientific reports 2022-10-29 [PMID: 36309544] (WB, Human)
  
  Details:
  Dilution used in WB 1:1000

Bioinformatics

• Gene Symbol: SPHK1
• Uniprot:
  • Q9NYA1()