Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Sphingosine Kinase 1/SPHK1 Antibody - BSA Free [NB110-40449] - Analysis of anti-SPHK1 antibody with human adrenal, pheochromocytes at concentration 10 ug/ml.

• Reactivity: Hu, Pm, Bt, Ca, Pm
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Sphingosine Kinase 1/SPHK1 Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 15 amino acid peptide from N-terminus of human Sphingosine Kinase 1/SPHK1.
• Epitope: N-terminal domain of human
• Localization: Cytoplasmic
• Specificity: Human Sphingosine Kinase 1/SPHK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Primate (93%), Canine (93%), Bat (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPHK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
• Application Notes: .

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gibbon (100%), Gorilla (93%), Equine (87%), Mouse (80%), Rat (80%), Bovine (80%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

• EC 2.7.1.91
• SK1
• sphingosine kinase 1
• SPHK1
• SPHKSK 1
• SPK 1
• SPK
• SPK1

Background

Sphingosine kinase (SPHK) phosphorylates sphingosine to form a bioactive lipid mediate, sphingosine 1-phosphate (S1P or SPP). S1P is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival while extracellularly it is a ligand for EDG 1. Expression of SPHK1 and subsequent production of S1P promotes the G1-S transition, protects cells from apoptosis, stimulates migration and angiogenesis and induces tumor formation in mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sphingosine Kinase 1/SPHK1 Antibody (NB110-40449)(3)

Publications using NB110-40449

• Hanker LC, El-Balat A, Drosos Z et al. Sphingosine-kinase-1 expression is associated with improved overall survival in high-grade serous ovarian cancer Journal of cancer research and clinical oncology 2021-03-03 [PMID: 33660008] (IHC-P, Human)
• Ruckhaberle E, Karn T, Denkert C et al. Predictive value of sphingosine kinase 1 expression in neoadjuvant treatment of breast cancer. J Cancer Res Clin Oncol. 2013-10-01 [PMID: 23955546]
• Ruckhaberle E, Rody A, Engels K et al. Microarray analysis of altered sphingolipid metabolism reveals prognostic significance of sphingosine kinase 1 in breast cancer. Breast Cancer Res Treat. 2008-11-01 [PMID: 18058224] (IHC-P, Human)
  
  Details:
  IHC (paraffin), human breast tissue, Fig. 9A,B.

Bioinformatics

• Gene Symbol: SPHK1