Sphingosine Kinase 1/SPHK1 Antibody (JA31-14)

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Western blot analysis of Sphingosine Kinase 1/SPHK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: MCF-7 cell lysate

Immunohistochemistry-Paraffin: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Analysis of paraffin-embedded human kidney tissue using anti-SPHK1 antibody. Counter stained with hematoxylin.

Flow Cytometry: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Analysis of Raji cells with SPHK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Analysis of SPHK1 on C2C12 lysates using anti-SPHK1 antibody at 1/1,000 dilution.

• Reactivity: Hu
• Applications: WB, Flow, IHC
• Clone: JA31-14
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Sphingosine Kinase 1/SPHK1 aa 25-74 / 384. (SwissProt: Q9NYA1 Human)
• Localization: Cytoplasm. Nucleus. Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SPHK1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Sphingosine Kinase 1/SPHK1 Antibody (JA31-14)

• EC 2.7.1.91
• SK1
• sphingosine kinase 1
• SPHK1
• SPHKSK 1
• SPK 1
• SPK
• SPK1

Background

Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPHK1