PIAS2 Antibody

Images

 
Western Blot: PIAS2 Antibody [NBP1-52022] - WB analysis of PIAS2 using anti-PIAS2 antibody on Jurkat cell lyste (30 ug cell lysate boiled in lysis buffer). Image from verified customer review.
Western Blot: PIAS2 Antibody [NBP1-52022] - Western blot analysis of PIAS2 (aa185-196) in Human Peripheral Lymphocytes lysate (35ug protein in RIPA buffer) using NBP1-52022 at 1ug/ml. Primary incubation was 1 hour. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

PIAS2 Antibody Summary

Immunogen
Peptide with sequence PQQVREICISRD corresponding to internal region according to NP_004662.2.
Epitope
PQQVREICISRD
Specificity
This antibody is expected to recognize both reported isoforms (NP_775298.1; NP_004662.2).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PIAS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 60 kDa band observed in human spleen and peripheral mononucleocytes lysates (calculated MW of 63.4 kDa band according to NP_775298.1).
Reviewed Applications
Read 1 Review rated 4
using
NBP1-52022 in the following applications:

Publications
Read Publication using NBP1-52022.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PIAS2 Antibody

  • Androgen receptor-interacting protein 3
  • ARIP3
  • DAB2-interacting protein
  • DIP
  • E3 SUMO-protein ligase PIAS2
  • MGC102682
  • miz
  • MIZ1
  • Msx-interacting zinc finger protein
  • Msx-interacting-zinc finger
  • PIAS2
  • PIAS-NY protein
  • PIASx alpha
  • PIASX
  • PIASX-ALPHA
  • PIASX-BETA
  • Protein inhibitor of activated STAT x
  • protein inhibitor of activated STAT, 2
  • Protein inhibitor of activated STAT2
  • SIZ2
  • zinc finger, MIZ-type containing 4
  • ZMIZ4

Background

This gene encodes a protein involved in the regulation of transcription factors involved in MAP kinase signaling. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. Two alternatively spliced transcripts encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PIAS2 Antibody (NBP1-52022)(1)

Review for PIAS2 Antibody (NBP1-52022) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-52022:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot PIAS2 NBP1-52022
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reviewed by:
Anonymous
WB Human 10/02/2014
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Summary

ApplicationWestern Blot
Sample TestedJurkat cells
SpeciesHuman

Blocking

Blocking DetailsBSA (5%) in PBST

Primary Anitbody

Dilution Ratio1:500 in PBST buffer

Secondary Antibody

Secondary DescriptionRabbit anti-Goat IgG Antibody (HRP)-Novus
Secondary Manufacturer Cat#Cat No NB710-H
Secondary ConcentrationDiluted 5,000 times

Details

Detection NotesDetected using PromoKine ECL Chemiluminescence Detection Kit with a 2 min exposure on X-ray film

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PIAS2 Antibody (NBP1-52022) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for PIAS2 Antibody (NBP1-52022)

Discover related pathways, diseases and genes to PIAS2 Antibody (NBP1-52022). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on PIAS2

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Recent Reviews

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5
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4
1
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2
0
1
0

Anonymous
10/02/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol PIAS2
Entrez
Uniprot