SPEN Antibody

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Immunoprecipitation: SPEN Antibody [NB100-58799] - Detection of Human SHARP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

       

Order Details

SPEN Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 3375 and 3425 of human SMART/HDAC1 associated repressor protein (spen homolog, transcriptional regulator) using the numbering given in entry NP_055816.2 (GeneID 23013).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SPEN
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 2 - 5 ug/mg lysate
Application Notes
Immunoprecipitation - 2 to 5 mcg/mg lysate. Western Blot - Not recommended. Immunohisto-/cytochemistry - Not tested.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SPEN Antibody

  • HIAA0929
  • KIAA0929nuclear receptor transcription cofactor
  • MINTMsx2 interacting nuclear target (MINT) homolog
  • RBM15C
  • SHARPmsx2-interacting protein
  • SMART/HDAC1 associated repressor protein
  • SMART/HDAC1-associated repressor protein
  • SPEN homolog
  • spen homolog, transcriptional regulator (Drosophila)

Background

This gene encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics Tool for SPEN Antibody (NB100-58799)

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Bioinformatics

Gene Symbol SPEN
Entrez
Uniprot