SPEN Antibody - BSA Free

Immunoprecipitation: SPEN Antibody [NB100-58799] - Detection of Human SHARP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SHARP antibody NB100-58799 used for IP at 3 mcg/mg lysate. SHARP was also immunoprecipitated by rabbit anti-SHARP antibody NB100-58798, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu
• Applications: WB, Func, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SPEN Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SPEN Antibody - BSA Free (NB100-58799) is a polyclonal antibody validated for use in WB, IP and ChIP. Anti-SPEN Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 3375 and 3425 of human SMART/HDAC1 associated repressor protein (spen homolog, transcriptional regulator) using the numbering given in entry NP_055816.2 (GeneID 23013).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPEN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 2-5 ug/mg lysate
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Proximity Ligation Assay Reported in scientific literature (PMID: 29880719).
  • Western Blot Reported in scientific literature (PMID: 29880719).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29880719).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SPEN Antibody - BSA Free

• HIAA0929
• KIAA0929nuclear receptor transcription cofactor
• MINTMsx2 interacting nuclear target (MINT) homolog
• RBM15C
• SHARPmsx2-interacting protein
• SMART/HDAC1 associated repressor protein
• SMART/HDAC1-associated repressor protein
• SPEN homolog
• spen homolog, transcriptional regulator (Drosophila)

Background

This gene encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SPEN Antibody (NB100-58799)(2)

Publications using NB100-58799

• Ho JJD, Cunningham TA, Manara P Et al. Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Cell reports 2021-10-12 [PMID: 34644561] (WB)
• Korfanty J, Stokowy T, Chadalski M et al. SPEN protein expression and interactions with chromatin in mouse testicular cells Reproduction 2018-06-07 [PMID: 29880719] (PLA, ChIP, Mouse)

Bioinformatics

• Gene Symbol: SPEN
• Entrez:
  • 23013(Human)
• Uniprot:
  • NP_055816.2(Human)