SPEN Antibody [DyLight 550]

• Reactivity: Hu, Mu
• Applications: WB, Func, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 550

SPEN Antibody [DyLight 550] Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 3375 and 3425 of human SMART/HDAC1 associated repressor protein (spen homolog, transcriptional regulator) using the numbering given in entry NP_055816.2 (GeneID 23013).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPEN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunoprecipitation
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29880719).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for SPEN Antibody [DyLight 550]

• HIAA0929
• KIAA0929nuclear receptor transcription cofactor
• MINTMsx2 interacting nuclear target (MINT) homolog
• RBM15C
• SHARPmsx2-interacting protein
• SMART/HDAC1 associated repressor protein
• SMART/HDAC1-associated repressor protein
• SPEN homolog
• spen homolog, transcriptional regulator (Drosophila)

Background

SPEN encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPEN