Spectrin beta 1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Spectrin beta 1 Antibody [NBP1-87596] - Staining of human cell line U-2 OS shows localization to cytosol & the Golgi apparatus.

Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody [NBP1-87596] - Analysis in human skeletal muscle and liver tissues using NBP1-87596 antibody. Corresponding Spectrin beta 1 RNA-seq data are presented for the same tissues.

Immunohistochemistry: Spectrin beta 1 Antibody [NBP1-87596] - Staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.

Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody [NBP1-87596] - Staining of human prostate shows low expression as expected.

Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody [NBP1-87596] - Staining of human skeletal muscle shows high expression.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Spectrin beta 1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: ESTKDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQLMDSHPEQKEDIGQRQKHLEELWQGLQQSLQGQEDLLGEVSQLQAFLQDLDDFQAWLSITQKAVASEDMPESL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPTB
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, PFA/Triton X-100.
• Control Peptide:

  Spectrin beta 1 Protein (NBP1-87596PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Spectrin beta 1 Antibody - BSA Free

• beta-I spectrin
• EL3
• erythrocyte
• HS2
• HSPTB1
• membrane cytoskeletal protein
• spectrin, beta, erythrocytic
• SPTB1

Background

Spectrin (Sp), the most abundant of the erythrocyte membrane skeleton proteins, helps these cells maintain their characteristic biconcave shape while remaining flexible and elastic. Erythrocyte Sp is a heterodimer composed of a 280 kDa alpha subunit and a 246 kDa beta subunit which associate in a side-to-side, antiparallel configuration to form a 100 nm rod-like structure. Sp in other tissues may be composed of distinct but homologous alpha and beta subunits, sometimes referred to as fodrin. A newly introduced nomenclature designates the Sp subunits of the erythrocyte as alpha-1 and beta-1, and the fodrin subunits as alpha-2 and beta-2. Alternatively spliced forms of each are designated as epsilon-1, epsilon-2, etc. (e.g. beta-1 epsilon-1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Spectrin beta 1 Antibody (NBP1-87596)(1)

Publication using NBP1-87596

• Huang CY, Zhang C, Ho TS et al. aII spectrin forms a periodic cytoskeleton at the axon initial segment and is required for nervous system function. J. Neurosci. 2017-10-16 [PMID: 29038240] (Human)

Bioinformatics

• Gene Symbol: SPTB