EPB41 Antibody (3D9)

Western Blot: EPB41 Antibody (3D9) [H00002035-M01] - Analysis of EPB41 expression in transfected 293T cell line by EPB41 monoclonal antibody (M01), clone 3D9.Lane 1: EPB41 transfected lysate(81 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: EPB41 Antibody (3D9) [H00002035-M01] - Analysis of monoclonal antibody to EPB41 on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: EPB41 Antibody (3D9) [H00002035-M01] - Analysis of monoclonal antibody to EPB41 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.

Sandwich ELISA: EPB41 Antibody (3D9) [H00002035-M01] - Detection limit for recombinant GST tagged EPB41 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
• Clone: 3D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

EPB41 Antibody (3D9) Summary

• Immunogen: EPB41 (AAH39079, 116 a.a. ~ 225 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IEFGTSLDEEIILKAPIAAPEPELKTDPSLDLHSLSSAETQPAQEELREDPDFEIKEGEGLEECSKIEVKEESPQSKAETELKASQKPIRKHRNMHCKVSLLDDTVYECV
• Specificity: EPB41 - erythrocyte membrane protein band 4.1 (elliptocytosis 1, RH-linked)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EPB41
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate for WB. It has been used for IHC-P, IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EPB41 Antibody (3D9)

• 4.1RBand 4.1
• E41P
• EL1
• EPB4.1
• erythrocyte membrane protein band 4.1 (elliptocytosis 1, RH-linked)
• erythrocyte surface protein band 4.1
• HE
• P4.1
• protein 4.1

Background

Elliptocytosis is a hematologic disorder characterized by elliptically shaped erythrocytes and a variable degree of hemolytic anemia. Inherited as an autosomal dominant, elliptocytosis results from mutation in any one of several genes encoding proteins of the red cell membrane skeleton. The form discussed here is the one found in the 1950s to be linked to Rh blood group and more recently shown to be caused by a defect in protein 4.1. 'Rh-unlinked' forms of elliptocytosis are caused by mutation in the alpha-spectrin gene (MIM 182860), the beta-spectrin gene (MIM 182870), or the band 3 gene (MIM 109270).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EPB41
• Entrez:
  • 2035(Human)
• OMIM:
  • 130500(Human)
• Uniprot:
  • AAH39079(Human)