SP1 Antibody - BSA Free

Immunohistochemistry-Paraffin: SP1 Antibody [NBP2-20460] - SP1 antibody detects SP1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. SP1 stained by SP1 antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: SP1 Antibody [NBP2-20460] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunoprecipitation: SP1 Antibody [NBP2-20460] - Immunoprecipitation of SP1 protein from THP-1 whole cell extracts using 5 ug of OCT1 antibody (NBP2-20460). Western blot analysis was performed using OCT1 antibody (NBP2-20460). EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Immunocytochemistry/ Immunofluorescence: SP1 Antibody [NBP2-20460] - SP1 antibody detects SP1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SP1 stained by SP1 antibody (NBP2-20460) diluted at 1:100.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .

Western Blot: SP1 Antibody [NBP2-20460] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (NBP2-20460) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: SP1 Antibody [NBP2-20460] - SP1 antibody detects SP1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SP1 stained by SP1 antibody (NBP2-20460) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.

Immunohistochemistry-Paraffin: SP1 Antibody [NBP2-20460] - SP1 antibody detects SP1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.SP1 stained by SP1 antibody (NBP2-20460) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (NBP2-20460) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using SP1(NBP2-20460) antibody at 1:500 dilution. br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

ChIP was performed with 293T chromatin extract and 5 ug of either normal rabbit IgG or anti-SP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to MGARP promoter.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB, ICC/IF, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SP1 Antibody - BSA Free (NBP2-20460) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, IP and ChIP. Anti-SP1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SP1. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Bovine (98%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SP1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Application Notes: Chip-Assay dependent
• Theoretical MW: 81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for SP1 Antibody - BSA Free

• Sp1 transcription factor
• specificity protein 1
• transcription factor Sp1
• TSFP1

Background

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SP1 Antibody (NBP2-20460)(2)

Publications using NBP2-20460

• Zhang H, Gao Y, Liu B et al. The activation of M (3) muscarinic receptor reverses liver injuryvia the Sp1/lncRNA Gm2199/miR-212 axis Acta Biochimica et Biophysica Sinica 2022-09-25 [PMID: 36111745] (Chemotaxis, Immunoprecipitation, Western Blot, Rat)
• Zhang Z, Zhang X, Wang C Et al. Deacetylated Sp1 improves bets glycerophosphate induced calcification in vascular smooth muscle cells Experimental and Therapeutic Medicine 2021-08-10 [PMID: 34504597] (IP, Chemotaxis, WB, Rat)

Bioinformatics

• Gene Symbol: SP1
• Uniprot:
  • P08047(Human)