SP1 Antibody


Western Blot: SP1 Antibody [NBP2-20460] - Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: SP1 Antibody [NBP2-20460] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SP1 protein stained by SP1 antibody diluted at 1:500. Red: phalloidin, a ...read more
Immunohistochemistry-Paraffin: SP1 Antibody [NBP2-20460] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.
Chromatin Immunoprecipitation (ChIP): SP1 Antibody [NBP2-20460] - ChIP was performed with 293T chromatin extract and 5 ug of either normal rabbit IgG or anti-SP1 antibody. The precipitated DNA was detected by PCR with ...read more
Western Blot: SP1 Antibody [NBP2-20460] - Sample (30 ug of whole cell lysate) A: THP-1 5% SDS PAGE gel, diluted at 1:2000.
Immunohistochemistry-Paraffin: SP1 Antibody [NBP2-20460] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.
Immunoprecipitation: SP1 Antibody [NBP2-20460] - Sample: 1000 ug HeLa whole cell lysate/extract A. 50 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of SP1 protein ...read more
Immunoprecipitation: SP1 Antibody [NBP2-20460] - Immunoprecipitation of SP1 protein from THP-1 whole cell extracts using 5 ug of OCT1 antibody (NBP2-20460). Western blot analysis was performed using OCT1 antibody ...read more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, ChIP
0.29 mg/ml

Order Details

SP1 Antibody Summary

Recombinant fragment corresponding to a region within amino acids 18 and 303 of SP1 (Uniprot ID#P08047)
Predicted Species
Rat (99%), Bovine (98%), Monkey (99%)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
1XPBS, 20% Glycerol (pH7).
0.025% Proclin 300
0.29 mg/ml
Immunogen affinity purified


Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. ChIP assay Assay-dependent dilution
Theoretical MW
81 kDa

Reactivity Notes

Rhesus Monkey (99%).

Alternate Names for SP1 Antibody

  • Sp1 transcription factor
  • specificity protein 1
  • transcription factor Sp1
  • TSFP1


Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

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Blogs on SP1.

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Nur77 is a member of the steroid/thyroid hormone phosphoprotein receptor superfamily. It is heavily post-translationally modified and rapidly induced in response to androgens and growth factors. It governs fundamental processes such as cell proliferat...  Read full blog post.

Estrogen Related Receptors Play Roles in Cancer and Neurodegeneration
By Eric NeeleyEstrogen receptors come in the form of two distinct forms, ER alpha and ER beta. These nuclear receptors are predominantly activated by the hormone 17-beta-estradiol to control transcription of genes throughout the immune, nervous, car...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol SP1

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