SP1 Antibody


Genetic Strategies: Western Blot: SP1 Antibody [NBP2-20460] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 ...read more
Immunocytochemistry/ Immunofluorescence: SP1 Antibody [NBP2-20460] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SP1 protein stained by SP1 antibody diluted at 1:500. Red: phalloidin, a ...read more
Immunohistochemistry-Paraffin: SP1 Antibody [NBP2-20460] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.
Chromatin Immunoprecipitation: SP1 Antibody [NBP2-20460] - ChIP was performed with 293T chromatin extract and 5 ug of either normal rabbit IgG or anti-SP1 antibody. The precipitated DNA was detected by PCR with primer ...read more
Western Blot: SP1 Antibody [NBP2-20460] - Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody diluted at 1:2000.
Immunohistochemistry-Paraffin: SP1 Antibody [NBP2-20460] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.
Immunoprecipitation: SP1 Antibody [NBP2-20460] - Immunoprecipitation of SP1 protein from THP-1 whole cell extracts using 5 ug of OCT1 antibody (NBP2-20460). Western blot analysis was performed using OCT1 antibody ...read more

Product Details

Reactivity Hu, Mu, Rt, Bv, RMSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP, ChIP
Validated by:

Genetic Strategies


Order Details

SP1 Antibody Summary

Recombinant protein encompassing a sequence within the center region of human SP1. The exact sequence is proprietary.
Predicted Species
Bovine (98%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:500-1:3000
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Chromatin Immunoprecipitation (ChIP)
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7.0) and 20% Glycerol
0.025% Proclin 300
Immunogen affinity purified

Alternate Names for SP1 Antibody

  • Sp1 transcription factor
  • specificity protein 1
  • transcription factor Sp1
  • TSFP1


Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

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By Eric NeeleyEstrogen receptors come in the form of two distinct forms, ER alpha and ER beta. These nuclear receptors are predominantly activated by the hormone 17-beta-estradiol to control transcription of genes throughout the immune, nervous, car...  Read full blog post.

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Gene Symbol SP1