SP1 Antibody

Images

 
Western Blot: SP1 Antibody [NBP2-20460] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody. HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: SP1 Antibody [NBP2-20460] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SP1 protein stained by SP1 antibody diluted at 1:500. Red: phalloidin, a ...read more
Immunohistochemistry-Paraffin: SP1 Antibody [NBP2-20460] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.
Chromatin Immunoprecipitation (ChIP): SP1 Antibody [NBP2-20460] - ChIP was performed with 293T chromatin extract and 5 ug of either normal rabbit IgG or anti-SP1 antibody. The precipitated DNA was detected by PCR with ...read more
Western Blot: SP1 Antibody [NBP2-20460] - Sample (30 ug of whole cell lysate) A: THP-1 5% SDS PAGE gel, diluted at 1:2000.
Western Blot: SP1 Antibody [NBP2-20460] - Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody diluted at 1:2000.
Immunohistochemistry-Paraffin: SP1 Antibody [NBP2-20460] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.
Immunoprecipitation: SP1 Antibody [NBP2-20460] - Sample: 1000 ug HeLa whole cell lysate/extract A. 50 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of SP1 protein ...read more
Immunoprecipitation: SP1 Antibody [NBP2-20460] - Immunoprecipitation of SP1 protein from THP-1 whole cell extracts using 5 ug of OCT1 antibody (NBP2-20460). Western blot analysis was performed using OCT1 antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.29 mg/ml
     

Genetic Strategies

   

Order Details

SP1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SP1. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (99%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
0.29 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SP1 Antibody

  • Sp1 transcription factor
  • specificity protein 1
  • transcription factor Sp1
  • TSFP1

Background

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for SP1 Antibody (NBP2-20460) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SP1 Antibody (NBP2-20460)

Discover related pathways, diseases and genes to SP1 Antibody (NBP2-20460). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on SP1.

Nur77 Activation and Tumor Suppression
Nur77 is a member of the steroid/thyroid hormone phosphoprotein receptor superfamily. It is heavily post-translationally modified and rapidly induced in response to androgens and growth factors. It governs fundamental processes such as cell proliferat...  Read full blog post.

Estrogen Related Receptors Play Roles in Cancer and Neurodegeneration
By Eric NeeleyEstrogen receptors come in the form of two distinct forms, ER alpha and ER beta. These nuclear receptors are predominantly activated by the hormone 17-beta-estradiol to control transcription of genes throughout the immune, nervous, car...  Read full blog post.

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Bioinformatics

Gene Symbol SP1
Uniprot