SOX4 Antibody (CL5634)

Images

 
Immunocytochemistry/ Immunofluorescence: SOX4 Antibody (CL5634) [NBP2-61420] - Staining of SH-SY5Y cells using the Anti-SOX4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and ...read more
Immunohistochemistry-Paraffin: SOX4 Antibody (CL5634) [NBP2-61420] - Staining of rat hippocampus shows strong nuclear immunoreactivity in a subset of cells in the granule cell layer of the dentate gyrus.
Immunohistochemistry-Paraffin: SOX4 Antibody (CL5634) [NBP2-61420] - Staining of human glioma shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: SOX4 Antibody (CL5634) [NBP2-61420] - Staining of human liver shows absence of nuclear positivity in hepatocytes as expected (negative control).
Immunohistochemistry-Paraffin: SOX4 Antibody (CL5634) [NBP2-61420] - Staining of mouse forebrain shows strong positivity in cells in the rostral migratory stream, as well as in a subset of neurons in the olfactory bulb.
Immunohistochemistry-Paraffin: SOX4 Antibody (CL5634) [NBP2-61420] - Staining of mouse forebrain shows strong immunoreactivity in cells in the rostral migratory stream.
Immunohistochemistry-Paraffin: SOX4 Antibody (CL5634) [NBP2-61420] - Staining of mouse hippocampus shows strong immunoreactivity in a subset of neurons in the granule cell layer of the dentate gyrus.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clone
CL5634
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SOX4 Antibody (CL5634) Summary

Immunogen
This antibody was developed against a synthetic peptide corresponding to amino acids: AGADPSDPLGLYEEEGAG
Specificity
Specificity of human SOX4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SOX4
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: PFA/Triton X-100

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for SOX4 Antibody (CL5634)

  • ecotropic viral integration site 16
  • EVI16
  • SRY (sex determining region Y)-box 4
  • SRY-related HMG-box gene 4
  • transcription factor SOX-4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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By Jamshed Arslan Pharm.D. Gastric carcinoma is the second leading cause of cancer-related deaths worldwide. One of the key features of gastric carcinoma is acidosis, which promotes growth and metastasis of gastric...  Read full blog post.

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Bioinformatics

Gene Symbol SOX4