SOX11 Antibody (CL0142) - Azide and BSA Free

Staining of eye in mouse embryo E14 retina shows moderate nuclear positivity in developing retina.

Staining of human chronic lymphocytic leukemia shows no nuclear positivity in tumor cells as expected.

Staining of human mantle cell lymphoma shows moderate to strong nuclear positivity in tumor cells.

Staining of mouse embryo E14 shows strong nuclear positivity in the developing forebrain.

Analysis in control (vector only transfected HEK293T lysate) and sOX11 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) .

Staining of RH-30 cells using the Anti-SOX11 monoclonal antibody) .

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF, IHC
• Clone: CL0142
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

SOX11 Antibody (CL0142) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse SOX11 Antibody (CL0142) - Azide and BSA Free (NBP2-14782) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P48431, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOX11
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Simple Western 1:20
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100
  See Simple Western Antibody Database for Simple Western validation: Tested in U-251MG sp and SHSY5Y, separated by Size, antibody dilution of 1:20, apparent MW was 53 kDa

Reactivity Notes

Expected to cross react based on sequence identity: Rat (82%). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at -20C prior to opening. Aliquot reconstituted liquid and and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for SOX11 Antibody (CL0142) - Azide and BSA Free

• SOX11
• SRY (sex determining region Y)-box 11
• SRY (sex-determining region Y)-box 11
• SRY-related HMG-box gene 11
• transcription factor SOX-11

Background

SOX11 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The protein may function in the developing nervous system and play a role in tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOX11