POU3F3 Antibody

Western Blot: POU3F3 Antibody [NBP1-49872] - Staining of Mouse Spinal Cord (A) and Brain (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: POU3F3 Antibody [NBP1-49872] - Immunofluorescence analysis of paraformaldehyde fixed Neuro-2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Flow Cytometry: POU3F3 Antibody [NBP1-49872] - Flow cytometric analysis of paraformaldehyde fixed Neuro-2a cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Mu, Hu, Rt
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

POU3F3 Antibody Summary

• Description: Novus Biologicals Goat POU3F3 Antibody (NBP1-49872) is a polyclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-POU3F3 Antibody: Cited in 10 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-HMLSHAHQWVTAL corresponding to internal region according to NP_006227.1.
• Epitope: HMLSHAHQWVTAL - internal region
• Predicted Species: Human (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: POU3F3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 1 - 3 ug/mL
• Application Notes: IHC-Fr reactivity reported in (PMID: 25273085). IHC reactivity reported in (PMID: 25224226).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for POU3F3 Antibody

• brain-1
• Brain-specific homeobox/POU domain protein 1
• Brn-1
• BRN1brn-1
• Oct-8
• Octamer-binding protein 8
• Octamer-binding transcription factor 8
• OTF-8
• OTF8oct-8
• POU class 3 homeobox 3
• POU domain class 3, transcription factor 3
• POU domain, class 3, transcription factor 3

Background

POU3F3 is a member of the class III POU family of transcription factors (see POU3F1; MIM 602479) that are expressed in the central nervous system. The POU domain in these proteins is required for high affinity binding to octamer DNA sequences Sumiyama et al. (1996) [PubMed 8703082].[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for POU3F3 Antibody (NBP1-49872)(11)

Publications using NBP1-49872

• Schnell, J;Miao, Z;Achieng, M;Fausto, CC;Koppitch, K;Takhirov, L;Wang, V;De Kuyper, F;Huang, B;Schreiber, M;Medina, P;Thornton, ME;Grubbs, B;Li, Z;Kim, J;Lindström, NO; Controlling nephron precursor differentiation to generate proximal-biased kidney organoids with emerging maturity Nature communications 2025-08-30 [PMID: 40885711]
• Salamon I, Park Y, Mi�ki? T et al. Celf4 controls mRNA translation underlying synaptic development in the prenatal mammalian neocortex Nat Commun 2023-09-27 [PMID: 37758766]
• Iva Salamon, Geeta Palsule, Xiaobing Luo, Alfonso Roque, Shawn Tucai, Ishan Khosla, Nicole Volk, Wendy Liu, Huijuan Cui, Valentina Dal Pozzo, Petronio Zalamea, Xinfu Jiao, Gabriella D'Arcangelo, Ronald P Hart, Mladen-Roko Rasin, Megerditch Kiledjian mRNA-Decapping Associated DcpS Enzyme Controls Critical Steps of Neuronal Development. Cerebral cortex (New York, N.Y. : 1991) 2022-04-05 [PMID: 34467373]
• Lindstrom NO, Sealfon R, Chen X et al. Spatial Transcriptional Mapping of the Human Nephrogenic Program Dev Cell 2021-08-24 [PMID: 34428401]
• Boitnott A, Ung D, Garcia-Forn M, et al. Developmental and behavioral phenotypes in a new mouse model of DDX3X syndrome Biol Psychiatry 2021-08-04 [PMID: 34344536]
• Braun S, Petrova R, Tang J et al. The npBAF to nBAF Chromatin Switch Regulates Cell Cycle Exit in the Developing Mammalian Cortex Genes Dev 2021-02-19 [PMID: 33602870]
• Abhay P. S. Rathore, Wilfried A. A. Saron, Ting Lim, Nusrat Jahan, Ashley L. St. John Maternal immunity and antibodies to dengue virus promote infection and Zika virus–induced microcephaly in fetuses Science Advances 2019-02-01 [PMID: 30820456]
• Zahr SK, Yang G, Kazan H, A Translational Repression Complex in Developing Mammalian Neural Stem Cells that Regulates Neuronal Specification. Neuron. 2018-02-07 [PMID: 29395907]
• Ka M, Condorelli G, Woodgett JR, Kim WY. mTOR regulates brain morphogenesis by mediating GSK3 signaling. Development. 2014-11-01 [PMID: 25273085] (IHC-Fr, Mouse)
  
  Details:
  POU3F3 / Brn1 antibody used for IHC of brain sections from control and mTorloxP/loxP; Nestin-cre mice at E15.5 stage (Fig. 5A)
• Ka M, Jung EM, Mueller U, Kim WY. MACF1 regulates the migration of pyramidal neurons via microtubule dynamics and GSK-3 signaling. Dev. Biol. 2014-09-16 [PMID: 25224226] (IF/IHC, Mouse)
• Castro DS, Skowronska-Krawczyk D, Armant O et al. Proneural bHLH and Brn proteins coregulate a neurogenic program through cooperative binding to a conserved DNA motif. Dev Cell 2006-12-01 [PMID: 17141158]

Review for POU3F3 Antibody (NBP1-49872) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Woo-Yang Kim IF Mouse 01/12/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse Brain Tissue
• Species: Mouse

Bioinformatics

• Gene Symbol: POU3F3
• Entrez:
  • 5455(Human)
  • 18993(Mouse)
  • 192109(Rat)
• Uniprot:
  • NP_006227.1(Human)