POU3F3 Antibody

Western Blot: POU3F3 Antibody [NBP1-49872] - Staining of Mouse Spinal Cord (A) and Brain (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: POU3F3 Antibody [NBP1-49872] - Immunofluorescence analysis of paraformaldehyde fixed Neuro-2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Flow Cytometry: POU3F3 Antibody [NBP1-49872] - Flow cytometric analysis of paraformaldehyde fixed Neuro-2a cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Mu, Hu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

POU3F3 Antibody Summary

• Immunogen: Peptide with sequence C-HMLSHAHQWVTAL corresponding to internal region according to NP_006227.1.
• Epitope: HMLSHAHQWVTAL - internal region
• Predicted Species: Human (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: POU3F3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 1 - 3 ug/mL
• Application Notes: IHC-Fr reactivity reported in (PMID: 25273085). IHC reactivity reported in (PMID: 25224226).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for POU3F3 Antibody

• brain-1
• Brain-specific homeobox/POU domain protein 1
• Brn-1
• BRN1brn-1
• Oct-8
• Octamer-binding protein 8
• Octamer-binding transcription factor 8
• OTF-8
• OTF8oct-8
• POU class 3 homeobox 3
• POU domain class 3, transcription factor 3
• POU domain, class 3, transcription factor 3

Background

POU3F3 is a member of the class III POU family of transcription factors (see POU3F1; MIM 602479) that are expressed in the central nervous system. The POU domain in these proteins is required for high affinity binding to octamer DNA sequences Sumiyama et al. (1996) [PubMed 8703082].[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for POU3F3 Antibody (NBP1-49872)(5)

Publications using NBP1-49872

• Boitnott A, Ung D, Garcia-Forn M, et al. Developmental and behavioral phenotypes in a new mouse model of DDX3X syndrome Biol Psychiatry 2021-08-04 [PMID: 34344536]
• Braun S, Petrova R, Tang J et al. The npBAF to nBAF Chromatin Switch Regulates Cell Cycle Exit in the Developing Mammalian Cortex Genes Dev 2021-02-19 [PMID: 33602870]
• Ka M, Condorelli G, Woodgett JR, Kim WY. mTOR regulates brain morphogenesis by mediating GSK3 signaling. Development. 2014-11-01 [PMID: 25273085] (IHC-Fr, Mouse)
  
  Details:
  POU3F3 / Brn1 antibody used for IHC of brain sections from control and mTorloxP/loxP; Nestin-cre mice at E15.5 stage (Fig. 5A)
• Ka M, Jung EM, Mueller U, Kim WY. MACF1 regulates the migration of pyramidal neurons via microtubule dynamics and GSK-3 signaling. Dev. Biol. 2014-09-16 [PMID: 25224226] (IHC, Mouse)
• Castro DS, Skowronska-Krawczyk D, Armant O et al. Proneural bHLH and Brn proteins coregulate a neurogenic program through cooperative binding to a conserved DNA motif. Dev Cell 2006-12-01 [PMID: 17141158]

Review for POU3F3 Antibody (NBP1-49872) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IF Mouse 01/12/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse Brain Tissue
• Species: Mouse

Bioinformatics

• Gene Symbol: POU3F3
• Entrez:
  • 5455(Human)
  • 18993(Mouse)
  • 192109(Rat)
• Uniprot:
  • NP_006227.1(Human)