POU3F3 Antibody

Images

 
Western Blot: POU3F3 Antibody [NBP1-49872] - Staining of Mouse Spinal Cord (A) and Brain (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: POU3F3 Antibody [NBP1-49872] - Immunofluorescence analysis of paraformaldehyde fixed Neuro-2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed ...read more
Flow Cytometry: POU3F3 Antibody [NBP1-49872] - Flow cytometric analysis of paraformaldehyde fixed Neuro-2a cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more

Product Details

Summary
Reactivity Mu, Hu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

POU3F3 Antibody Summary

Immunogen
Peptide with sequence C-HMLSHAHQWVTAL corresponding to internal region according to NP_006227.1.
Epitope
HMLSHAHQWVTAL - internal region
Predicted Species
Human (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
POU3F3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/mL
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Peptide ELISA Detection limit 1:4000
Application Notes
IHC-Fr reactivity reported in (PMID: 25273085). IHC reactivity reported in (PMID: 25224226).
Reviewed Applications
Read 1 Review rated 5
using
NBP1-49872 in the following applications:

Publications
Read Publications using
NBP1-49872 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for POU3F3 Antibody

  • brain-1
  • Brain-specific homeobox/POU domain protein 1
  • Brn-1
  • BRN1brn-1
  • Oct-8
  • Octamer-binding protein 8
  • Octamer-binding transcription factor 8
  • OTF-8
  • OTF8oct-8
  • POU class 3 homeobox 3
  • POU domain class 3, transcription factor 3
  • POU domain, class 3, transcription factor 3

Background

POU3F3 is a member of the class III POU family of transcription factors (see POU3F1; MIM 602479) that are expressed in the central nervous system. The POU domain in these proteins is required for high affinity binding to octamer DNA sequences Sumiyama et al. (1996) [PubMed 8703082].[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for POU3F3 Antibody (NBP1-49872)(5)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC, IHC-Fr.


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IHC
(2)
IHC-Fr
(1)
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Mouse
(3)
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Review for POU3F3 Antibody (NBP1-49872) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-49872:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence POU3F3 NBP1-49872
Enlarge
5
reviewed by:
Woo-Yang Kim
IF Mouse 01/12/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse Brain Tissue
SpeciesMouse

Blocking

Blocking DetailsBlocking Buffer: 5% goat serum, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution Ratio1: 500-1000

Details

Detection NotesDetection Method: epifluorescence; Sample Preparation: OCT embedded Cryosection
Fixation DetailsPFA fixation; Permeabilization using Triton X-100; PBS or TBS buffers

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for POU3F3 Antibody (NBP1-49872) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for POU3F3 Antibody (NBP1-49872)

Discover related pathways, diseases and genes to POU3F3 Antibody (NBP1-49872). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Woo-Yang Kim
01/12/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol POU3F3
Entrez
Uniprot