SOX1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of undifferentiated iBJ6 human iPS cells and iBJ6 human iPS cells differentiated into neuroprogenitor cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOX1 ...read more
Simple Western lane view shows lysates of undifferentiated iBJ6 human iPS cells andiBJ6 human iPS cells differentiated into neuroprogenitor cells, loaded at 0.2 mg/mL. A specific band was detected for SOX1 at ...read more
SOX1 was detected in immersion fixed rat cortical stem cells (Catalog # NSC001) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room ...read more
SOX1 was detected in immersion fixed mouse cortical stem cells (Catalog # NSC002) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room ...read more
SOX1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neural progenitor cells using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at ...read more
SOX1 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line differentiated with retinoic acid using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

SOX1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOX1
Asn242-Gly379 (Leu276Ile)
Accession # NP_005977
Specificity
Detects human SOX1 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human (rh) SOX2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOX1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF3369 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX1 Antibody [Unconjugated]

  • SOX1
  • SRY (sex determining region Y)-box 1
  • SRY-related HMG-box gene 1
  • transcription factor SOX-1

Background

SOX1 is a 39 kDa transcription factor that belongs to the SOXB1 subgroup. Within the developing CNS, SOX1 maintains neural cells in an undifferentiated state and has been used as a marker for neural stem cells. Human and mouse SOX1 share 97% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOX1 Antibody (AF3369)(36)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 5 applications: ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ICC
(25)
IHC
(7)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(25)
Mouse
(8)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using AF3369 Applications Species
V Meraviglia, CH Arendzen, M Tok, C Freund, AS Maione, E Sommariva, M Bellin Generation of human induced pluripotent stem cell line LUMCi027-A and its isogenic gene-corrected line from a patient affected by arrhythmogenic cardiomyopathy and carrying the c.2013delC PKP2 mutation Stem Cell Res, 2020;46(0):101835. 2020 [PMID: 32485643] (ICC, Human) ICC Human
V Meraviglia, CH Arendzen, C Freund, DE Atsma, CL Mummery, M Bellin Generation of two human induced pluripotent stem cell lines, LUMCi020-A and LUMCi021-A, from two patients with Catecholaminergic Polymorphic Ventricular Tachycardia carrying heterozygous mutations in the RYR2 gene Stem Cell Res, 2020;45(0):101764. 2020 [PMID: 32315959] (ICC, Human) ICC Human
N Bredenkamp, J Yang, J Clarke, GG Stirparo, F von Meyenn, S Dietmann, D Baker, R Drummond, Y Ren, D Li, C Wu, M Rostovskay, S Eminli-Mei, A Smith, G Guo Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency Stem Cell Reports, 2019;13(6):1083-1098. 2019 [PMID: 31708477] (ICC, Human) ICC Human
P Wörsdörfer, N Dalda, A Kern, S Krüger, N Wagner, CK Kwok, E Henke, S Ergün Generation of complex human organoid models including vascular networks by incorporation of mesodermal progenitor cells Sci Rep, 2019;9(1):15663. 2019 [PMID: 31666641] (IHC-P, Human) IHC-P Human
B Di Stefano, EC Luo, C Haggerty, S Aigner, J Charlton, J Brumbaugh, F Ji, I Rabano Jim, KJ Clowers, AJ Huebner, K Clement, I Lipchina, MAC de Kort, A Anselmo, J Pulice, MFM Gerli, H Gu, SP Gygi, RI Sadreyev, A Meissner, GW Yeo, K Hochedling The RNA Helicase DDX6 Controls Cellular Plasticity by Modulating P-Body Homeostasis Cell Stem Cell, 2019;0(0):. 2019 [PMID: 31588046] (ICC, Human) ICC Human
S Park, JE Yoo, DR Lee, J Jang, MS Cho, DS Kim, DW Kim Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23) Stem Cell Res, 2019;39(0):101499. 2019 [PMID: 31352199] (ICC, Human) ICC Human
J Li, C Narayanan, J Bian, D Sambo, T Brickler, W Zhang, S Chetty A transient DMSO treatment increases the differentiation potential of human pluripotent stem cells through the Rb family PLoS ONE, 2018;13(12):e0208110. 2018 [PMID: 30540809] (ICC, Human) ICC Human
Y Kang, Z Ai, K Duan, C Si, Y Wang, Y Zheng, J He, Y Yin, S Zhao, B Niu, X Zhu, L Liu, L Xiang, L Zhang, Y Niu, W Ji, T Li Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys Cell Rep, 2018;25(9):2563-2576.e9. 2018 [PMID: 30485820] (ICC, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)) ICC Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
AM Lager, OG Corradin, JM Cregg, MS Elitt, HE Shick, BLL Clayton, KC Allan, HE Olsen, M Madhavan, PJ Tesar Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells Nat Commun, 2018;9(1):3708. 2018 [PMID: 30213958] (ICC, Mouse) ICC Mouse
AD Senft, I Costello, HW King, AW Mould, EK Bikoff, EJ Robertson Combinatorial Smad2/3 Activities Downstream of Nodal Signaling Maintain Embryonic/Extra-Embryonic Cell Identities during Lineage Priming Cell Rep, 2018;24(8):1977-1985.e7. 2018 [PMID: 30134160] (ICC, Human) ICC Human
Show All 36 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Blogs on SOX1.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

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Bioinformatics

Gene Symbol SOX1
Uniprot