SOX11 Antibody (CL0142) - BSA Free

Western Blot: SOX11 Antibody (CL0142) [NBP2-14782] - Analysis in control (vector only transfected HEK293T lysate) and sOX11 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: SOX11 Antibody (CL0142) [NBP2-14782] - Staining of RH-30 cells using the Anti-SOX11 monoclonal antibody, showing specific staining in the nucleus and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.

Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP2-14782] - Staining of eye in mouse embryo E14 retina shows moderate nuclear positivity in developing retina.

Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP2-14782] - Staining of human chronic lymphoid leukemia shows no positivity in tumor cells.

Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP2-14782] - Staining of human mantle cell lymphoma shows moderate to strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP2-14782] - Staining of mouse embryo E14 shows nuclear immunoreactivity in the developing forebrain neurons.

Simple Western: SOX11 Antibody (CL0142) [NBP2-14782] - Simple Western lane view shows a specific band for SOX11 in 0.2 mg/ml of U-251MG sp (left) and SHSY5Y (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF, IHC
• Clone: CL0142
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

SOX11 Antibody (CL0142) - BSA Free Summary

• Description: Novus Biologicals Mouse SOX11 Antibody (CL0142) - BSA Free (NBP2-14782) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from P48431, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOX11
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Simple Western 5 ug/ml
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in U-251MG sp and SHSY5Y, separated by Size, antibody dilution of 5 ug/mL, apparent MW was 53 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reactivity Notes

Expected to cross react based on sequence identity: Rat (82%). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for SOX11 Antibody (CL0142) - BSA Free

• SOX11
• SRY (sex determining region Y)-box 11
• SRY (sex-determining region Y)-box 11
• SRY-related HMG-box gene 11
• transcription factor SOX-11

Background

SOX11 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The protein may function in the developing nervous system and play a role in tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOX11