SOX10 Antibody (SOX10/991 + SOX10/1074)

Western Blot: SOX10 Antibody (SOX10/991 + SOX10/1074) [NBP2-44476] - (A) Recombinant protein (B) A375 Cell Lysate using SOX10 Monoclonal Antibody (SOX10/991 + SOX10/1074).

Immunohistochemistry-Paraffin: SOX10 Antibody (SOX10/991 + SOX10/1074) [NBP2-44476] - Human Melanoma stained with SOX10 Monoclonal Antibody (SOX10/991 + SOX10/1074).

Flow Cytometry: SOX10 Antibody (SOX10/991 + SOX10/1074) [NBP2-44476] - Flow Cytometry: SOX10 Antibody (SOX10/991 + SOX10/1074) [Alexa Fluor® 488] [NBP2-47709AF488] - An intracellular stain was performed on SK-MEL-28 cells with SOX10 Antibody (SOX10/991 + SOX10/1074) NBP2-47709AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.

Product Discontinued

SOX10 Antibody (SOX10/991 + SOX10/1074) Summary

• Immunogen: Recombinant fragment (aa 115-269) of human SOX10 protein with hexa-histidine tag
• Localization: Nuclear
• Marker: Melanoma Marker
• Specificity: Recognizes a protein of ~55kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOX10
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunofluorescence 1 - 2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Western Blot 0.5 - 1.0 ug/ml
• Application Notes: Hu-chromosome location: 22q13.1
  Molecular weight of antigen: 49-58kDa
  Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10mM PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for SOX10 Antibody (SOX10/991 + SOX10/1074)

• DOM
• MGC15649
• PCWH
• SOX10
• SOX-10
• SRY (sex determining region Y)-box 10
• SRY-related HMG-box gene 10
• transcription factor SOX-10
• WS2E
• WS4
• WS4C
• WS4mouse, human homolog of

Background

SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. It acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOX10 Antibody (NBP2-44476)(1)

Publication using NBP2-44476

• Ye D, Wang Q, Yang Y et al. Identifying Genes that Affect Differentiation of Human Neural Stem Cells and Myelination of Mature Oligodendrocytes Cellular and molecular neurobiology 2022-12-22 [PMID: 36547781]

Bioinformatics

• Gene Symbol: SOX10
• Uniprot:
  • P56693()