SORBS1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: SORBS1 Antibody [NBP1-86641] - Staining of human cell line U-2 OS shows localization to plasma membrane, centrosome & focal adhesion sites. Antibody staining is shown in green.

Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SORBS1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Staining of human endometrium, gastrointestinal, heart muscle and testis using Anti-SORBS1 antibody NBP1-86641 (A) shows similar protein distribution across tissues to independent antibody NBP2-33383 (B).

Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.

Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Staining of human colon shows strong cytoplasmic positivity in glandular cells.

Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SORBS1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: DSSPLLNEVSSSLIGTDSQAFPSVSKPSSAYPSTTIVNPTIVLLQHNREQQKRLSSLSDPVSERRVGEQDSAPTQEKPTSPGKAIEKRAKDDSRRVVKSTQDLSDVSMDEVGIPLRNTERSKDWYKTMFKQIHKLNR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SORBS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SORBS1 Protein (NBP1-86641PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (85%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SORBS1 Antibody - BSA Free

• c-Cbl associated protein
• c-Cbl-associated protein
• DKFZp451C066
• DKFZp586P1422
• Fas-ligand associated factor 2
• FLAF2
• KIAA0894
• KIAA1296CAPSH3D5SH3P12FLJ12406
• ponsin
• R85FL
• SH3 domain protein 5
• SH3-domain protein 5 (ponsin)
• sh3p12
• SORB1
• sorbin and SH3 domain containing 1
• sorbin and SH3 domain-containing protein 1

Background

SORBS1 plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SORBS1 Antibody (NBP1-86641)(2)

Publications using NBP1-86641

• Brock K, Alpha KM, Brennan G, De Jong EP et Al. A comparative analysis of paxillin and Hic-5 proximity interactomes Cytoskeleton (Hoboken) 2024-05-27 [PMID: 38801098]
• Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261]

Bioinformatics

• Gene Symbol: SORBS1