Sonic Hedgehog/Shh Antibody (5H4)

Images

 
Western Blot: Sonic Hedgehog Antibody (5H4) [NBP2-22126] - Western blot analysis using Sonic Hedgehog mouse mAb against LNCaP (1), HepG2 (2), PANC-1 (3),HeLa (4), SK-N-SH (5), F9 (6), NIH3T3 (7), and COS7 (8) cell ...read more
Immunohistochemistry-Paraffin: Sonic Hedgehog Antibody (5H4) [NBP2-22126] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using Sonic Hedgehog mouse mAb with DAB staining.
Flow Cytometry: Sonic Hedgehog Antibody (5H4) [NBP2-22126] - Flow cytometric analysis of HeLa cells using Sonic Hedgehog mouse mAb (green) and negative control (red).
Western Blot: Sonic Hedgehog Antibody (5H4) [NBP2-22126] - Western blot analysis using Sonic Hedgehog mAb against human Sonic Hedgehog recombinant protein. (Expected MW is 41 kDa)
Western Blot: Sonic Hedgehog Antibody (5H4) [NBP2-22126] - Western blot analysis using Sonic Hedgehog mAb against HEK293 (1) and Sonic Hedgehog (AA: 26-161)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: Sonic Hedgehog Antibody (5H4) [NBP2-22126] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ELISA, Flow, IHC-P
Clone
5H4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Sonic Hedgehog/Shh Antibody (5H4) Summary

Immunogen
Purified recombinant fragment of human Sonic Hedgehog (amino acids 26-170) expressed in E. coli. [UniProt# Q15465]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SHH
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This Sonic Hedgehog (5H4) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry and ELISA.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
49.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-22126 in the following applications:

Reactivity Notes

Human, mouse and monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Sonic Hedgehog/Shh Antibody (5H4)

  • HHG1
  • HHG-1
  • HLP3
  • HPE3
  • MCOPCB5sonic hedgehog (Drosophila) homolog
  • Shh
  • SMMCIsonic hedgehog homolog (Drosophila)
  • sonic hedgehog homolog
  • sonic hedgehog protein
  • Sonic Hedgehog
  • TPT
  • TPTPS

Background

This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Sonic Hedgehog/Shh Antibody (NBP2-22126)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(3)
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Filter By Species
Human
(3)
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Bioinformatics

Gene Symbol SHH
Entrez
Uniprot