Sonic Hedgehog/Shh Antibody (171018) [Unconjugated] - N-Terminus

Recombinant Mouse Sonic Hedgehog N‑Terminus (Catalog # 461-SH or 464-SH) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Mouse Sonic Hedgehog N‑Terminus (5 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Sonic Hedgehog N‑Terminus Monoclonal Antibody (Catalog # MAB4641). The ND50 is typically 5-15 µg/mL.

Western blot shows lysates of human brain tissue, human stomach tissue, mouse brain tissue, and mouse stomach tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody (Catalog # MAB4641) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Sonic Hedgehog/Shh was detected in immersion fixed frozen sections of mouse embryo (11 d.p.c.) using Rat Anti-Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody (Catalog # MAB4641) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC, B/N, ELISA(Cap)
• Clone: 171018
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Sonic Hedgehog/Shh Antibody (171018) [Unconjugated] - N-Terminus Summary

• Immunogen: E. coli-derived recombinant mouse Sonic Hedgehog
  Cys25-Gly198 (Lys122Arg)
  Accession # Q62226
• Specificity: Detects mouse Sonic Hedgehog in ELISAs. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Dhh
  (N‑terminus; aa 23‑198) is observed and no cross-reactivity with rmIhh (N-terminus; aa 66‑240), rmDhh (C‑terminus; aa 199‑396), or rmShh (C‑terminus; aa 199-437) is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: SHH
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 8-25 ug/mL
  • Neutralization 5-15 ug/mL
  • Western Blot 2 ug/mL
• Application Notes: ELISA Detection: Mouse Sonic Hedgehog/Shh N-Terminus Biotinylated Antibody (Catalog number BAM4642)
  Standard: Recombinant Mouse Sonic Hedgehog/Shh N-Terminus (Catalog number 461-SH)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sonic Hedgehog/Shh Antibody (171018) [Unconjugated] - N-Terminus

• HHG1
• HHG-1
• HLP3
• HPE3
• MCOPCB5
• MCOPCB5sonic hedgehog (Drosophila) homolog
• Shh
• ShhNC
• SMMCI
• SMMCIsonic hedgehog homolog (Drosophila)
• sonic hedgehog homolog
• sonic hedgehog protein
• Sonic Hedgehog
• TPT
• TPTPS

Background

Sonic Hedgehog (Shh) is a hedgehog protein that is instrumental in patterning the early embryo. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sonic Hedgehog/Shh Antibody (MAB4641)(9)

Publications using MAB4641

• Christopher J. Tape, Stephanie Ling, Maria Dimitriadi, Kelly M. McMahon, Jonathan D. Worboys, Hui Sun Leong et al. Oncogenic KRAS Regulates Tumor Cell Signaling via Stromal Reciprocation Cell 5/5/2016 [PMID: 27087446]
• WOO JEAN KIM, SAE-WON LEE, KYU-WON KIM Sonic hedgehog secreted by neurons regulates angiopoietin expression in neighboring fibroblasts International Journal of Molecular Medicine 7/1/2014 [PMID: 24807580]
• Engevik A, Feng R, Yang L, Zavros Y The acid-secreting parietal cell as an endocrine source of Sonic Hedgehog during gastric repair. Endocrinology, 2013-10-03;154(12):4627-39. 2013-10-03 [PMID: 24092639] (Neutralization, Mouse)
• Céline Delloye-Bourgeois, Benjamin Gibert, Nicolas Rama, Jean-Guy Delcros, Nicolas Gadot, Jean-Yves Scoazec et al. Sonic Hedgehog Promotes Tumor Cell Survival by Inhibiting CDON Pro-Apoptotic Activity PLoS Biology 8/6/2013 [PMID: 23940460]
• Céline Delloye-Bourgeois, Benjamin Gibert, Nicolas Rama, Jean-Guy Delcros, Nicolas Gadot, Jean-Yves Scoazec et al. Sonic Hedgehog Promotes Tumor Cell Survival by Inhibiting CDON Pro-Apoptotic Activity PLoS Biology 2013-08-06 [PMID: 23940460] (ELISA Capture, Mouse)
• Mohamad El-Zaatari, Stephanie Daignault, Art Tessier, Gail Kelsey, Lisa A. Travnikar, Esperanza F. Cantu et al. Plasma Shh Levels Reduced in Pancreatic Cancer Patients Pancreas 10/1/2012 [PMID: 22513293]
• Mohamad El-Zaatari, Stephanie Daignault, Art Tessier, Gail Kelsey, Lisa A. Travnikar, Esperanza F. Cantu et al. Plasma Shh Levels Reduced in Pancreatic Cancer Patients Pancreas 2012-10-01 [PMID: 22513293] (ELISA Capture, Human)
• Kazumasa Nakamura, Junpei Sasajima, Yusuke Mizukami, Yoshiaki Sugiyama, Madoka Yamazaki, Rie Fujii et al. Hedgehog Promotes Neovascularization in Pancreatic Cancers by Regulating Ang-1 and IGF-1 Expression in Bone-Marrow Derived Pro-Angiogenic Cells PLoS ONE 1/21/2010 [PMID: 20098680]
• Mfopou JK, De Groote V, Xu X, Heimberg H, Bouwens L Sonic hedgehog and other soluble factors from differentiating embryoid bodies inhibit pancreas development. Stem Cells, 2007-02-01;25(5):1156-65. 2007-02-01 [PMID: 17272496] (ELISA Development, Neutralization, Mouse)

Bioinformatics

• Gene Symbol: SHH
• Uniprot:
  • Q62226()