Sonic Hedgehog/Shh Antibody (171018) [Alexa Fluor® 750]

• Reactivity: Mu
• Applications: WB, IHC, B/N, ELISA(Cap), ELISA(Det)
• Clone: 171018
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 750

Sonic Hedgehog/Shh Antibody (171018) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant mouse Sonic Hedgehog
  Cys25-Gly198 (Lys122Arg)
  Accession # Q62226
• Specificity: Detects mouse Sonic Hedgehog in ELISAs. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Dhh
  (N‑terminus; aa 23‑198) is observed and no cross-reactivity with rmIhh (N-terminus; aa 66‑240), rmDhh (C‑terminus; aa 199‑
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • Immunohistochemistry
  • Neutralization
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sonic Hedgehog/Shh Antibody (171018) [Alexa Fluor® 750]

• HHG1
• HHG-1
• HLP3
• HPE3
• MCOPCB5
• MCOPCB5sonic hedgehog (Drosophila) homolog
• Shh
• ShhNC
• SMMCI
• SMMCIsonic hedgehog homolog (Drosophila)
• sonic hedgehog homolog
• sonic hedgehog protein
• Sonic Hedgehog
• TPT
• TPTPS

Background

Sonic Hedgehog (Shh) is a hedgehog protein that is instrumental in patterning the early embryo. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.