SOD2/Mn-SOD Antibody (8H3D2) - BSA Free

Western Blot: SOD2/Mn-SOD Antibody (8H3D2) [NBP2-61883] - Analysis using SOD2 mAb against human SOD2 (AA: 1-222) recombinant protein. (Expected MW is 50.7 kDa)

Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody (8H3D2) [NBP2-61883] - Analysis of Hela cells using SOD2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry: SOD2/Mn-SOD Antibody (8H3D2) [NBP2-61883] - Analysis of paraffin-embedded rectum cancer tissues using SOD2 mouse mAb with DAB staining.

Flow Cytometry: SOD2/Mn-SOD Antibody (8H3D2) [NBP2-61883] - Analysis of Hela cells using SOD2 mouse mAb (green) and negative control (red).

Western Blot: SOD2/Mn-SOD Antibody (8H3D2) [NBP2-61883] - Analysis using SOD2 mAb against HEK293 (1) and SOD2 (AA: 1-222)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry: SOD2/Mn-SOD Antibody (8H3D2) [NBP2-61883] - Analysis of paraffin-embedded ovarian cancer tissues using SOD2 mouse mAb with DAB staining.

ELISA: SOD2/Mn-SOD Antibody (8H3D2) [NBP2-61883] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 8H3D2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SOD2/Mn-SOD Antibody (8H3D2) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human SOD2/Mn-SOD (AA: 1-222) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOD2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 24.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for SOD2/Mn-SOD Antibody (8H3D2) - BSA Free

• EC 1.15.1.1
• indophenoloxidase B
• IPOB
• IPO-B
• manganese-containing superoxide dismutase
• mangano-superoxide dismutase
• Mn SOD
• Mn superoxide dismutase
• MnSOD
• MVCD6
• SOD, Mitochodrial
• SOD2
• superoxide dismutase [Mn], mitochondrial
• superoxide dismutase 2, mitochondrial

Background

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOD2