SOD1/Cu-Zn SOD Antibody (JF1005)

Western Blot: SOD1/Cu-Zn SOD Antibody (JF1005) [NBP2-67158] - Analysis of SOD1 on MCF-7 cells lysates using anti-SOD1 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: SOD1/Cu-Zn SOD Antibody (JF1005) [NBP2-67158] - Staining SOD1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody (JF1005) [NBP2-67158] - Analysis of paraffin-embedded rat brain tissue using anti-SOD1 antibody. Counter stained with hematoxylin.

Flow Cytometry: SOD1/Cu-Zn SOD Antibody (JF1005) [NBP2-67158] - Analysis of Jurkat cells with SOD1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: SOD1/Cu-Zn SOD Antibody (JF1005) [NBP2-67158] - Staining SOD1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: SOD1/Cu-Zn SOD Antibody (JF1005) [NBP2-67158] - Staining SOD1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody (JF1005) [NBP2-67158] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-SOD1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody (JF1005) [NBP2-67158] - Analysis of paraffin-embedded human liver tissue using anti-SOD1 antibody. Counter stained with hematoxylin.

Product Discontinued

SOD1/Cu-Zn SOD Antibody (JF1005) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human SOD1/Cu-Zn SOD aa 105-154 / 154. (SwissProt: P00441 Human; SwissProt: P07632 Rat)
• Localization: Cytoplasm, Mitochondrion, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SOD1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SOD1/Cu-Zn SOD Antibody (JF1005)

• ALS
• ALS1
• amyotrophic lateral sclerosis 1 (adult)
• Cu
• Cu/Zn superoxide dismutase
• CuZn SOD
• Cu-Zn SOD
• EC 1.15.1.1
• homodimer
• hSod1
• indophenoloxidase A
• Ipo1
• IPOA
• SOD
• SOD, cytosolic
• SOD, Soluble
• SOD1
• superoxide dismutase [Cu-Zn]
• Superoxide dismutase 1
• superoxide dismutase 1, soluble
• Zn superoxide dismutase, EC 1.15.1.110superoxide dismutase, cystolic

Background

FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. CATALYTIC ACTIVITY: 2 superoxide + 2 H+ = O2 + H2O2. COFACTOR: Binds 1 copper ion per subunit. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. DISEASE: Defects in SOD1 are the cause of familial amyotrophic lateral sclerosis (FALS); also called amyotrophic lateral sclerosis 1 (ALS1 or ALS). ALS is a degenerative disorder of motorneurons in the cortex, brainstem and spinal cord. ALS is characterized by muscular weakness and atrophy beginning in the hands and spreading to the forearms and legs. Muscle fasciculations are commonly visible. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. ALS is sometimes referred to as Lou Gehrig disease after the famous American baseball player who was diagnosed with the disorder. FALS, the familial form of ALS, accounts for about 10% of the cases and is transmitted in an autosomal dominant manner. The mean age at onset of FALS is 45 years. MISCELLANEOUS: Zinc binding promotes dimerization. SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOD1/Cu-Zn SOD Antibody (NBP2-67158)(2)

Publications using NBP2-67158

• Sincihu Y, Lusno MFD, Mulyasari TM et al. Wistar Rats Hippocampal Neurons Response to Blood Low-Density Polyethylene Microplastics: A Pathway Analysis of SOD, CAT, MDA, 8-OHdG Expression in Hippocampal Neurons and Blood Serum A?42 Levels Neuropsychiatric disease and treatment 2023-01-05 [PMID: 36636141] (IHC-P, Rat)
• Pitsillidou C Identifying age related changes in the human immune system by using polychromatic flow cytometry Thesis 2023-01-01

Bioinformatics

• Gene Symbol: SOD1