SNAP25 Antibody (4E11)

Images

 
Western Blot: SNAP25 Antibody (4E11) [NBP1-04344] - The extracts of mouse brain (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human SNAP25 (1:2000). Proteins were visualized using a ...read more
Immunocytochemistry/ Immunofluorescence: SNAP25 Antibody (4E11) [NBP1-04344] - Analysis of SNAP25 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SNAP25 antibody (1:100) with ...read more
Flow Cytometry: SNAP25 Antibody (4E11) [NBP1-04344] - Analysis of SNAP25 in U87MG cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. ...read more
Western Blot: SNAP25 Antibody (4E11) [NBP1-04344] - The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:3000). Proteins were visualized using a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
4E11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

SNAP25 Antibody (4E11) Summary

Immunogen
Recombinant human SNAP25 (1-206aa) purified from E. coli
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SNAP25
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:3000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for SNAP25 Antibody (4E11)

  • bA416N4.2
  • dJ1068F16.2
  • FLJ23079
  • resistance to inhibitors of cholinesterase 4 homolog
  • RIC4
  • RIC-4
  • SEC9
  • SNAP25
  • SNAP-25SNAP
  • SUP
  • Super protein
  • Synaptosomal-associated 25 kDa protein
  • synaptosomal-associated protein 25
  • synaptosomal-associated protein, 25kD
  • synaptosomal-associated protein, 25kDa

Background

The synaptosomal-associated protein (SNAP25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP25 is directly involved in the release of neurotransmitters.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SNAP25 Antibody (NBP1-04344) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-04344AF350
Alexa Fluor 405 NBP1-04344AF405
Alexa Fluor 488 NBP1-04344AF488
Alexa Fluor 532 NBP1-04344AF532
Alexa Fluor 594 NBP1-04344AF594
Alexa Fluor 647 NBP1-04344AF647
Alexa Fluor 700 NBP1-04344AF700
Alexa Fluor 750 NBP1-04344AF750
Allophycocyanin NBP1-04344APC
Allophycocyanin/Cy7 NBP1-04344APCCY7
Biotin NBP1-04344B
DyLight 350 NBP1-04344UV
DyLight 405 NBP1-04344V
DyLight 488 NBP1-04344G
DyLight 550 NBP1-04344R
DyLight 594 NBP1-04344DL594
DyLight 650 NBP1-04344C
DyLight 680 NBP1-04344FR
DyLight 755 NBP1-04344IR
FITC NBP1-04344F
HRP NBP1-04344H
Janelia Fluor 549 NBP1-04344JF549
Janelia Fluor 646 NBP1-04344JF646
PE NBP1-04344PE
PE/Atto594 NBP1-04344PEATT594
PE/Cy5.5 NBP1-04344PECY55
PE/Cy7 NBP1-04344PECY7

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Characterizing Synaptophysin is "a Snap"
Synaptophysin is an integral membrane glycoprotein found within the small synaptic vesicles in brain and endocrine cells. Studies with synaptophysin antibodies show that it is one of the most abundant small vesicle proteins, constituting approximately...  Read full blog post.

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Bioinformatics

Gene Symbol SNAP25
Uniprot