SNAP25 Antibody (4A3)

Western Blot: SNAP25 Antibody (4A3) [H00006616-M01] - Analysis of SNAP25 expression in transfected 293T cell line by SNAP25 monoclonal antibody (M01), clone 4A3.Lane 1: SNAP25 transfected lysate(23.315 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: SNAP25 Antibody (4A3) [H00006616-M01] - Detection limit for recombinant GST tagged SNAP25 is 0.03 ng/ml as a capture antibody.

Product Discontinued

SNAP25 Antibody (4A3) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: SNAP25 (AAH10647, 31 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RMLQLVEESKDAGIRTLVMLDEQGEQLDRVEEGMNHINQDMKEAEKNLKDLGKCCGLFICPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAIS
• Specificity: SNAP25 - synaptosomal-associated protein, 25kDa
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SNAP25
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SNAP25 Antibody (4A3)

• bA416N4.2
• dJ1068F16.2
• FLJ23079
• resistance to inhibitors of cholinesterase 4 homolog
• RIC4
• RIC-4
• SEC9
• SNAP25
• SNAP-25SNAP
• SUP
• Super protein
• Synaptosomal-associated 25 kDa protein
• synaptosomal-associated protein 25
• synaptosomal-associated protein, 25kD
• synaptosomal-associated protein, 25kDa

Background

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SNAP25
• Entrez:
  • 6616(Human)
• OMIM:
  • 600322(Human)
• Uniprot:
  • AAH10647(Human)