Desert Hedgehog/Dhh Antibody [Unconjugated] - C-terminus

Immunohistochemistry of DHH, PTCH1 and PTCH2 in the tammar wallaby testis at key developmental time points. Red/brown staining indicates protein distribution while the heamatoxalin counterstain appears blue. It is important to note that DHH is a highly secreted molecule and staining does not imply cell of origin. DHH staining was most intense at the basal lamina (BL). In the adult staining is concentrated in the develop spermatocytes (GC). At day D9pp PTCH1 was present within the Sertoli cells (SC) while PTCH2 was predominant in the Leydig cells (LC). This expression profile is reversed in the adult testis with PTCH1 found predominantly in the Leydig cells, while PTCH2 was predominate in the Sertoli cells. Scale bars indicate 40 μm, controls show immunohistochemistry with the primary antibody omitted. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/22132805), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry of DHH, PTCH1 and PTCH2 in the tammar wallaby testis at key developmental time points. Red/brown staining indicates protein distribution while the heamatoxalin counterstain appears blue. It is important to note that DHH is a highly secreted molecule and staining does not imply cell of origin. DHH staining was most intense at the basal lamina (BL). In the adult staining is concentrated in the develop spermatocytes (GC). At day D9pp PTCH1 was present within the Sertoli cells (SC) while PTCH2 was predominant in the Leydig cells (LC). This expression profile is reversed in the adult testis with PTCH1 found predominantly in the Leydig cells, while PTCH2 was predominate in the Sertoli cells. Scale bars indicate 40 μm, controls show immunohistochemistry with the primary antibody omitted. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/22132805), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Desert Hedgehog/Dhh Antibody [Unconjugated] - C-terminus Summary

• Immunogen: E. coli-derived recombinant mouse Desert Hedgehog/Dhh C-Terminus
  Cys199-Gly396
  Accession # Q61488
• Specificity: Detects mouse Desert Hedgehog/Dhh C-Terminus in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant mouse (rm) Shh C‑terminus peptide (aa 199‑437) and rmIhh C-terminus peptide (aa 241‑449) is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DHH
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Desert Hedgehog/Dhh Antibody [Unconjugated] - C-terminus

• desert hedgehog (Drosophila) homolog
• desert hedgehog homolog
• desert hedgehog protein
• Desert Hedgehog
• DHH
• HHG-3MGC35145

Background

Desert Hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Hedgehogs are synthesized as 45 kDa precursors that are cleaved autocatalytically. The 19 kDa N-terminal fragment remains membrane associated due to its cholesterol and palmitate modifications. Binding of this fragment to Patched receptors results in the loss of Patched repression of Smoothened signaling (1‑4). Dhh binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip) (5). Within the N-terminal peptide, mouse Dhh shares 97% and 100% amino acid (aa) sequence identity with human and rat Dhh, respectively. It shares 74% aa seqeuence identity with mouse Indian (Ihh) and Sonic hedgehog (Shh) (6, 7). Dhh is produced by Sertoli cells and is required for testis development and spermatogenesis (8, 9). It induces steroidogenic factor 1 which is instrumental in promoting Leydig cell differentiation (10, 11). It also promotes the deposition of basal lamina surrounding seminiferous tubules (8). In humans, mutations of Dhh are associated with pure gonadal dysgenesis (12). Dhh is expressed in the female by ovarian granulosa cells and the corpus luteum (13). Its up‑regulation in human ovarian cancer correlates positively with proliferative index and negatively with prognosis (14). Dhh is also expressed by Schwann cells and is up‑regulated following nerve injury (15, 16). It induces the expression of Patched and Hip in nerve fibroblasts and promotes the formation of the connective tissue sheath surrounding peripheral nerves (15).

1. van den Brink, G.R. (2007) Physiol. Rev. 87:1343.
2. Riobo, N.A. and D.R. Manning (2007) Biochem. J. 403:369.
3. Porter, J.A. et al. (1995) Nature 374:363.
4. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. 95:13630.
5. Pathi, S. et al. (2001) Mech. Dev. 106:107.
6. Echelard, Y. et al. (1993) Cell 75:1417.
7. Chang, D.T. et al. (1994) Development 120:3339.
8. Pierucci-Alves, F. et al. (2001) Biol. Reprod. 65:1392.
9. Bitgood, M.J. et al. (1996) Curr. Biol. 6:298.
10. Yao, H.H.-C. et al. (2002) Genes Dev. 16:1433.
11. Park, S.Y. et al. (2007) Endocrinology 148:3704.
12. Canto, P. et al. (2004) J. Clin. Endocrinol. 89:4480.
13. Russell, M.C. et al. (2007) Biol. Reprod. 77:226.
14. Chen, X. et al. (2007) Cancer Sci. 98:68.
15. Parmantier, E. et al. (1999) Neuron 23:713.
16. Bajestan, S.N. et al. (2006) J. Neurobiol. 66:243.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Desert Hedgehog/Dhh Antibody (AF196)(3)

Publications using AF196

• William A O'Hara, Walid J Azar, Richard R Behringer, Marilyn B Renfree, Andrew J Pask Desert hedgehog is a mammal-specific gene expressed during testicular and ovarian development in a marsupial BMC Developmental Biology 2011-12-01 [PMID: 22132805] (Immunohistochemistry, Western Blot, Wallaby)
• William A O'Hara, Walid J Azar, Richard R Behringer, Marilyn B Renfree, Andrew J Pask Desert hedgehog is a mammal-specific gene expressed during testicular and ovarian development in a marsupial BMC Developmental Biology 12/1/2011 [PMID: 22132805]
• Sacedon R, Varas A, Hernandez-Lopez C, Gutierrez-deFrias C, Crompton T, Zapata AG, Vicente A Expression of hedgehog proteins in the human thymus. J. Histochem. Cytochem., 2003-11-01;51(11):1557-66. 2003-11-01 [PMID: 14566027] (IHC-Fr, Human)

Bioinformatics

• Gene Symbol: DHH
• Uniprot:
  • Q61488()