SMC4 Antibody - BSA Free

Western Blot: SMC4 Antibody [NB100-375] - Whole cell lysate (90 mcg for WB; 0.5 mg for IP/WB) from 293T cells (lanes 1, 3 and 5), normal human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Affinity purified rabbit anti-SMC4 used at 0.2 or 0.1 mcg/ml for WB and 5 mcg for IP.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SMC4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SMC4 Antibody - BSA Free (NB100-375) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-SMC4 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide, which represented a portion of human structural maintenance of chromosomes 4 encoded within exon 23 (LocusLink ID 10051). According to Swiss-Prot entry Q9NTJ3, the peptide is near the C-terminus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMC4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:400
  • Immunohistochemistry 1:200-1:400
  • Immunohistochemistry-Paraffin 1:200-1:400
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:1000-1:10000
• Application Notes: NB 100-375 may be used for Western Blot, where a band at ~170 kDa is seen, representing SMC4 protein.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SMC4 Antibody - BSA Free

• CAP-C
• CAPCSMC4 structural maintenance of chromosomes 4-like 1 (yeast)
• Chromosome-associated polypeptide C
• hCAP-CSMC4 (structural maintenance of chromosomes 4, yeast)-like 1
• SMC protein 4
• SMC4 structural maintenance of chromosomes 4-like 1
• SMC-4
• SMC4L1
• structural maintenance of chromosomes 4
• structural maintenance of chromosomes protein 4
• XCAP-C homolog

Background

Structural Maintenance of Chromosome (SMC) proteins are required for nuclear processes, including chromosome condensation, chromatid cohesion, and DNA repair. Eukaryotic proteins are capable of binding DNA and possess a DNA-stimulated ATPase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SMC4 Antibody (NB100-375)(2)

Publications using NB100-375

• Wood JL, Liang Y, Li K et al. Microcephalin/MCPH1 associates with the Condensin II complex to function in homologous recombination repair. J Biol Chem 2008-10-01 [PMID: 18718915]
• Kouznetsova A, Novak I, Jessberger R et al. SYCP2 and SYCP3 are required for cohesin core integrity at diplotene but not for centromere cohesion at the first meiotic division. J Cell Sci 2005-05-01 [PMID: 15870106]

Bioinformatics

• Gene Symbol: SMC4
• Uniprot:
  • Q9NTJ3(Human)