SMC5 Antibody

Western Blot: SMC5 Antibody [NB100-469] - Detection of human and mouse SMC5 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, RKO, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC5 antibody NB100-469 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: SMC5 Antibody [NB100-469] - Detection of human SMC5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC5 antibody NB100-469 (lot 3) used for IP at 6 ug per reaction. SMC5 was also immunoprecipitated by a previous lot of this antibody (lot 2). For blotting immunoprecipitated SMC5, NB100-469 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

SMC5 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 1050 and the C-terminus (residue 1101) of human Structural Maintenance of Chromosomes 5 using the numbering given in TrEMBL entry Q8IY18 (GeneID 23137).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMC5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000
• Application Notes: Use in ICC/IF reported in scientific literature (PMID 28302748).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SMC5 Antibody

• hSMC5
• KIAA0594SMC5 structural maintenance of chromosomes 5-like 1 (yeast)
• SMC protein 5
• SMC-5
• SMC5L1SMC5 structural maintenance of chromosomes 5-like 1
• structural maintenance of chromosomes 5
• structural maintenance of chromosomes protein 5

Background

SMC (Structural maintenance of chromosomes) proteins are members of multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. Two of these complexes, cohesin and condensin consist of a core complex of an SMC1 and SMC3 heterodimer or an SMC2 and SMC4 heterodimer, respectively. A third complex includes a heterodimer of SMC5 and SMC6 whose function is not fully understood but is proposed to be related to DNA repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SMC5 Antibody (NB100-469)(4)

Publications using NB100-469

• Venegas AB, Natsume T, Kanemaki M, Hickson ID Inducible Degradation of the Human SMC5/6 Complex Reveals an Essential Role Only during Interphase Cell Rep 2020-04-21 [PMID: 32320646] (WB, Human)
• Hwang G, Sun F, O'Brien M et al. SMC5/6 is required for the formation of segregation-competent bivalent chromosomes during meiosis I in mouse oocytes. Development. 2017-03-16 [PMID: 28302748] (ICC/IF, Mouse)
• Franklin S, Zhang MJ, Chen H et al. Expand+Specialized compartments of cardiac nuclei exhibit distinct proteomic anatomy. Mol Cell Proteomics. 2010-08-31 [PMID: 20807835]
• Draskovic I, Arnoult N, Steiner V et al. Probing PML body function in ALT cells reveals spatiotemporal requirements for telomere recombination. Proc Natl Acad Sci U S A. [PMID: 19717459]

Bioinformatics

• Gene Symbol: SMC5
• Entrez:
  • 23137(Human)
• Uniprot:
  • Q8IY18(Human)