SMC5 Antibody

Images

 
Western Blot: SMC5 Antibody [NB100-469] - Detection of human and mouse SMC5 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, RKO, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: ...read more
Immunoprecipitation: SMC5 Antibody [NB100-469] - Detection of human SMC5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

SMC5 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1050 and the C-terminus (residue 1101) of human Structural Maintenance of Chromosomes 5 using the numbering given in TrEMBL entry Q8IY18 (GeneID 23137).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMC5
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 2-10 ug/mg lysate
Application Notes
Use in ICC/IF reported in scientific literature (PMID 28302748).
Publications
Read Publications using
NB100-469 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SMC5 Antibody

  • hSMC5
  • KIAA0594SMC5 structural maintenance of chromosomes 5-like 1 (yeast)
  • SMC protein 5
  • SMC-5
  • SMC5L1SMC5 structural maintenance of chromosomes 5-like 1
  • structural maintenance of chromosomes 5
  • structural maintenance of chromosomes protein 5

Background

SMC (Structural maintenance of chromosomes) proteins are members of multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. Two of these complexes, cohesin and condensin consist of a core complex of an SMC1 and SMC3 heterodimer or an SMC2 and SMC4 heterodimer, respectively. A third complex includes a heterodimer of SMC5 and SMC6 whose function is not fully understood but is proposed to be related to DNA repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-469
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Publications for SMC5 Antibody (NB100-469)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SMC5 Antibody (NB100-469) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-469AF350
Alexa Fluor 405 NB100-469AF405
Alexa Fluor 488 NB100-469AF488
Alexa Fluor 532 NB100-469AF532
Alexa Fluor 594 NB100-469AF594
Alexa Fluor 647 NB100-469AF647
Alexa Fluor 700 NB100-469AF700
Alexa Fluor 750 NB100-469AF750
Biotin NB100-469B
DyLight 350 NB100-469UV
DyLight 405 NB100-469V
DyLight 488 NB100-469G
DyLight 550 NB100-469R
DyLight 594 NB100-469DL594
DyLight 650 NB100-469C
DyLight 680 NB100-469FR
DyLight 755 NB100-469IR
HRP NB100-469H
Janelia Fluor 549 NB100-469JF549
Janelia Fluor 646 NB100-469JF646

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Bioinformatics Tool for SMC5 Antibody (NB100-469)

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Contact Information

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Product PDFs

Calculators

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Bioinformatics

Gene Symbol SMC5
Entrez
Uniprot