SMC4 Antibody

Images

 
Western Blot: SMC4 Antibody [NB100-374] - Detection of Human SMC4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit ...read more
Immunocytochemistry/ Immunofluorescence: SMC4 Antibody [NB100-374] - HeLa cells grown on coverslips, fixed with methanol/acetone and blocked with 3% BSA in PBS. Antibody used at 5 ug/ml. Detection: Rhodamine labeled ...read more
Western Blot: SMC4 Antibody [NB100-374] - Whole cell lysate (90 ug) from 293T cells (lane 1), SMC4 transfected 239T cells (lane 2) or normal human lymphoblasts (lane 3) separated on an 8% SDS-PAGE gel. Antibody used ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

SMC4 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 600 and 650 of human Structural Maintenance of Chromosomes 4 using the numbering given in entry NP_005487.2 (GeneID 10051).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMC4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:2000
Application Notes
Immunofluorescence and Western Blot: The investigator should determine the optimal working dilution for a specific application.
Publications
Read Publication using NB100-374.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SMC4 Antibody

  • CAP-C
  • CAPCSMC4 structural maintenance of chromosomes 4-like 1 (yeast)
  • Chromosome-associated polypeptide C
  • hCAP-CSMC4 (structural maintenance of chromosomes 4, yeast)-like 1
  • SMC protein 4
  • SMC4 structural maintenance of chromosomes 4-like 1
  • SMC-4
  • SMC4L1
  • structural maintenance of chromosomes 4
  • structural maintenance of chromosomes protein 4
  • XCAP-C homolog

Background

Structural Maintenance of Chromosome (SMC) proteins are required for nuclear processes, including chromosome condensation, chromatid cohesion, and DNA repair. Eukaryotic proteins are capable of binding DNA and possess a DNA-stimulated ATPase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SMC4 Antibody (NB100-374)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SMC4 Antibody (NB100-374) (0)

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Secondary Antibodies

 

Isotype Controls

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Calculators

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Bioinformatics

Gene Symbol SMC4
Entrez
Uniprot