SMC1 Antibody

Images

 
Western Blot: SMC1 Antibody [NBP2-32142] - Samples: Whole cell lysate (50 ug) from 293T cells treated with 100 uM etoposide for 2 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-SMC1 antibody ...read more
Immunohistochemistry: SMC1 Antibody [NBP2-32142] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- Phospho SMC1 (S957) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. ...read more
Immunohistochemistry: SMC1 Antibody [NBP2-32142] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- Phospho SMC1 (S957) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. ...read more
Immunoprecipitation: SMC1 Antibody [NBP2-32142] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells treated with 100 uM etoposide for 2 hours (+) or mock treated (-) cells. Antibodies: SMC1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
   

Biological Strategies

     

Order Details

SMC1 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region of human SMC1 (structural maintenance of chromosomes 1-like 1 ) surrounding serine 957 using the numbering given in entry NP_006297.2 (Gene ID 8243) when the serine residue is phosphorylated.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMC1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • Immunohistochemistry
  • Immunoprecipitation 2 - 10 ug/mg lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SMC1 Antibody

  • CDLS2
  • DXS423ESMC1 structural maintenance of chromosomes 1-like 1 (yeast)
  • KIAA0178DKFZp686L19178
  • SB1.8
  • SB1.8MGC138332
  • segregation of mitotic chromosomes 1
  • SMC protein 1A
  • SMC1 (structural maintenance of chromosomes 1, yeast)-like 1
  • SMC1 structural maintenance of chromosomes 1-like 1
  • SMC1
  • SMC1A
  • SMC-1A
  • SMC-1-alpha
  • SMC1L1
  • SMC1SMC1alpha
  • Smcb
  • structural maintenance of chromosomes 1A
  • structural maintenance of chromosomes protein 1A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SMC1 Antibody (NBP2-32142). (Showing 1 - 2 of 2 FAQ).

  1. I would like to use one of your anti-smc1 antibodies to detect smc1 in an invertebrate. Your different antibodies are raised against different peptides derived from different regions possibly well-conserved, but not completely, in the organism that interests me (Schistosoma mansoni). Would it be possible to know the sequence of the different peptides that was used to generate the different antibodies, or failing that, could you analyze the corresponding sequence in my species of interest in order to determine whether or not it corresponds to one of the different peptides?
    • My suggestion to you would be to look at the homology between the protein expressed in your species and the protein expressed in the reactive species of the antibodies. A homology of >90% is a great start, >80% is acceptable. Please view our list of smc1 antibodies we currently offer and there are 27 products.
  2. My sequence of interest have >80% homology with the N-term and the C-term parts of human smc1. Your antibodies NB100-78322 and NB100-204 recognized the N-term and C-term of human smc1 respectively, therefore it’s possible that the cross reactivity can happen. I would be very interested to test these antibodies (NB100-78322 and NB100-204). Can you provide me free sample (few ul) to do western blot. I will share the testing result with you.
    • We do not provide free samples for testing. If you are interested in testing these antibodies in an unlisted species or application, you would qualify for the Innovator's Rewards program. The way the program works is that you would purchase the antibody of interest, perform your testing using appropriate controls and then share your data with us. Upon review of your data we would issue you a voucher for a free antibody. You can find more details here.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SMC1A
Entrez
Uniprot