SMC1 [p Ser966] Antibody

Images

 
Western Blot: SMC1 [p Ser966] Antibody [NB100-206] - Whole cell lysate (50 ug) from HeLa cells that were treated with NCS (+; 200 ng/ml; 1h) or mock treated (-). Antibody used at 0.1 ug/ml. For detection of total SMC1, ...read more
Immunocytochemistry/ Immunofluorescence: SMC1 [p Ser966] Antibody [NB100-206] - Neocarzinostatin treated asynchronous HeLa cells.
Immunohistochemistry: SMC1 [p Ser966] Antibody [NB100-206] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Phospho SMC1 (S966) used at a dilution of 1:1,000 (1ug/ml). ...read more
Western Blot: SMC1 [p Ser966] Antibody [NB100-206] - Analysis if human Phospho SMC1 (Ser966). Sample: Whole cell extracts from HeLa cells (30 ug/lane) that were irradiated with 10 Gy of ionizing radiation.
Proximity Ligation Assay: SMC1 [p Ser966] Antibody [NB100-206] - Secondary-conjugate Duolink II in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human phospho-SMC1 [S966] (NB100-206). Image merged from ...read more
Proximity Ligation Assay: SMC1 [p Ser966] Antibody [NB100-206] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM7 (NB100-252) and rabbit anti-human phospho-SMC1 [S966] (NB100-206). Image merged ...read more
Proximity Ligation Assay: SMC1 [p Ser966] Antibody [NB100-206] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MSH6 (NB100-328) and rabbit anti-human phospho-SMC1 [S966] (NB100-206). Image merged ...read more
Proximity Ligation Assay: SMC1 [p Ser966] Antibody [NB100-206] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC1 (NB100-230) and rabbit anti-human phospho-SMC1 [S966] (NB100-206). Image merged ...read more
Proximity Ligation Assay: SMC1 [p Ser966] Antibody [NB100-206] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SA1 (NB100-298) and rabbit anti-human phospho-SMC1 [S966] (NB100-206). Image merged ...read more
Proximity Ligation Assay: SMC1 [p Ser966] Antibody [NB100-206] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMC1 (NBP2-14858) and rabbit anti-human phospho-SMC1 [S966] (NB100-206). Image merged ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

SMC1 [p Ser966] Antibody Summary

Immunogen
Immunogen for this antibody was a phosphorylated synthetic peptide, which represented a portion of human Structural Maintenance of Chromosomes 1 (GeneID 8243) around serine 966 according to the numbering given in entry NP_006297.2.
Modification
p Ser966
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMC1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:250-1:1000
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2 - 6 ug/mg of lysate
  • Proximity Ligation Assay 1:1000-1:20000
Positive Control
SMC1 Lysate (NBL1-16236)
Reviewed Applications
Read 1 Review rated 5
using
NB100-206 in the following applications:

Publications
Read Publications using
NB100-206 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:25820262). Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Rhesus Monkey, White-tufted-ear marmoset, Crab-eating macaque, Small-eared galago and Northern white-cheeked gibbon.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-204

Alternate Names for SMC1 [p Ser966] Antibody

  • CDLS2
  • DXS423ESMC1 structural maintenance of chromosomes 1-like 1 (yeast)
  • KIAA0178DKFZp686L19178
  • SB1.8
  • SB1.8MGC138332
  • segregation of mitotic chromosomes 1
  • SMC protein 1A
  • SMC1 (structural maintenance of chromosomes 1, yeast)-like 1
  • SMC1 structural maintenance of chromosomes 1-like 1
  • SMC1
  • SMC1A
  • SMC-1A
  • SMC-1-alpha
  • SMC1L1
  • SMC1SMC1alpha
  • Smcb
  • structural maintenance of chromosomes 1A
  • structural maintenance of chromosomes protein 1A

Background

Structural maintenance of chromosomes (SMC) proteins are part of the cohesin and condensin multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. The core cohesin complex includes a heterodimer of SMC1 and SMC3 that functions by forming a ring-like structure that holds sister chromatids together. Cohesin is important to sister chromatid separation and segregation during mitosis and meiosis and also plays a role in double-strand break repair by homologous recombination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, PLA

Publications for SMC1 Antibody (NB100-206)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ELISA, ICC/IF, WB.


Filter By Application
ELISA
(1)
ICC/IF
(2)
WB
(4)
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Human
(5)
Mouse
(1)
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Showing Publications 1 - 9 of 9.
Publications using NB100-206 Applications Species
Batenburg NL, Thompson EL, Hendrickson EA, Zhu XD. Cockayne syndrome group B protein regulates DNA double-strand break repair and checkpoint activation. EMBO J. 2015 Mar 27 [PMID: 25820262] (WB, Mouse) WB Mouse
Lee P, Martin NT, Nakamura K et al. SMRT compounds abrogate cellular phenotypes of ataxia telangiectasia in neural derivatives of patient-specific hiPSCs. Nat Commun 2013 [PMID: 23652012] (ICC/IF, Human) ICC/IF Human
Du L, Kayali R, Bertoni C et al. Arginine-rich cell-penetrating peptide dramatically enhances AMO-mediated ATM aberrant splicing correction and enables delivery to brain and cerebellum. Hum Mol Genet;20(16):3151-60. 2011 Aug 15. [PMID: 21576124]
Takahashi A, Mori E, Ohnishi T. The foci of DNA double strand break-recognition proteins localize with gammaH2AX after heat treatment J Radiat Res 2010 [PMID: 20173316] (ICC/IF, Human) ICC/IF Human
Hu H, Du L, Nagabayashi G et al. ATM is down-regulated by N-Myc-regulated microRNA-421. Proc Natl Acad Sci U S A;107(4):1506-11. 2010 Jan 26. [PMID: 20080624] (WB, ELISA, Human) WB, ELISA Human
Nahas SA, Butch AW, Du L et al. Rapid Flow Cytometry-Based Structural Maintenance of Chromosomes 1 (SMC1) Phosphorylation Assay for Identification of Ataxia-Telangiectasia Homozygotes Heterozygotes. Clin Chem;55(3):463-472. 2009 [PMID: 19147735]
Leung-Pineda, Van et al. Phosphorylation of Chk1 by ATR Is Antagonized by a Chk1-Regulated Protein Phosphatase 2A Circuit. 26: 7529-7538. 2006 [PMID: 17015476]
Nakamura K, Fike F, Haghayegh S. A-TWinnipeg: Pathogenesis of rare ATM missense mutation c.6200C>A with decreased protein expression and downstream signaling, early-onset dystonia, cancer, and life-threatening radiotoxicity. Molecular Genetics & Genomic Medicine 2/2/2014 (WB, Human) WB Human
Nayler S, Gatei M, Kozlov S et al. Embryonic Stem Cells/Induced Pluripotent Stem (iPS) Cells: Induced Pluripotent Stem Cells from Ataxia-Telangiectasia Recapitulate the Cellular Phenotype Stem Cells Trans Med 2012 June (WB, Human) WB Human

Review for SMC1 Antibody (NB100-206) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-206:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot SMC1 NB100-206
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reviewed by:
Christie Brown
WB Human 08/15/2013
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Summary

ApplicationWestern Blot
Sample TestedLymphoblastoid (LCL) nuclear lysate
SpeciesHuman
LotA6
CommentsGreat antibody for WB. Strong, specific band after exposure to 10GY IR. Very clean with little/no background. I also use this antibody for flow cytometry.

Blocking

Blocking Details5% milk in PBSt

Primary Anitbody

Dilution Ratio1/3000, 2 hr incubation at RT, 5% milk in PBSt

Secondary Antibody

Secondary DescriptionDonkey anti Rabbit IgG, HRP
Secondary Manufacturer Cat#45-000-682
Secondary Concentration1/3000

Details

Detection NotesAmersham ECL, 5 min exposure, wild type and A-T cells

Comments

CommentsGreat antibody for WB. Strong, specific band after exposure to 10GY IR. Very clean with little/no background. I also use this antibody for flow cytometry.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SMC1 Antibody (NB100-206). (Showing 1 - 2 of 2 FAQs).

  1. I would like to use one of your anti-smc1 antibodies to detect smc1 in an invertebrate. Your different antibodies are raised against different peptides derived from different regions possibly well-conserved, but not completely, in the organism that interests me (Schistosoma mansoni). Would it be possible to know the sequence of the different peptides that was used to generate the different antibodies, or failing that, could you analyze the corresponding sequence in my species of interest in order to determine whether or not it corresponds to one of the different peptides?
    • My suggestion to you would be to look at the homology between the protein expressed in your species and the protein expressed in the reactive species of the antibodies. A homology of >90% is a great start, >80% is acceptable. Please view our list of smc1 antibodies we currently offer and there are 27 products.
  2. My sequence of interest have >80% homology with the N-term and the C-term parts of human smc1. Your antibodies NB100-78322 and NB100-204 recognized the N-term and C-term of human smc1 respectively, therefore it’s possible that the cross reactivity can happen. I would be very interested to test these antibodies (NB100-78322 and NB100-204). Can you provide me free sample (few ul) to do western blot. I will share the testing result with you.
    • We do not provide free samples for testing. If you are interested in testing these antibodies in an unlisted species or application, you would qualify for the Innovator's Rewards program. The way the program works is that you would purchase the antibody of interest, perform your testing using appropriate controls and then share your data with us. Upon review of your data we would issue you a voucher for a free antibody. You can find more details here.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Christie Brown
08/15/2013
Application: WB
Species: Human

Bioinformatics

Gene Symbol SMC1A
Entrez
Uniprot