SMARCA1 Antibody

Images

 
Western Blot: SMARCA1 Antibody [NB100-57524] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-57524 used for IP at 3 mcg/mg lysate.
Immunohistochemistry: SMARCA1 Antibody [NB100-57524] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SMARCA1/SNF2L used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: SMARCA1 Antibody [NB100-57524] - FFPE section of human colon carcinoma. Affinity purified rabbit anti-SMARCA1/SNF2L used at a dilution of 1:500.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

       

Order Details

SMARCA1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1004 and 1054 of human SMARCA1/SNF2L using the numbering given in entry NP_003060.2 (GeneID 6594).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMARCA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 mcg/mg
Application Notes
This antibody is not recommended for blotting crude sources of SMARCA1/SNF2L. NB100-57524 can be used at 1:2,000 to 1:10,000 for blotting enriched sources (e.g. immunoprecipitated and exogenously expressed) of SMARCA1/SNF2L.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SMARCA1 Antibody

  • ATP-dependent helicase SMARCA1
  • DKFZp686D1623
  • EC 3.6.1
  • EC 3.6.4.-
  • FLJ41547
  • global transcription activator homologous sequence
  • Nucleosome-remodeling factor subunit SNF2L
  • NURF140
  • probable global transcription activator SNF2L1
  • SNF2L
  • SNF2L1ISWI
  • SNF2LB
  • SNF2-like 1
  • SNF2LT
  • subfamily a, member 1
  • sucrose nonfermenting 2-like protein 1
  • SWI
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin
  • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a1
  • SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 1
  • SWI2

Background

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. Member of this family have helicase and ATPase activities that alter chromatin structure to facilitate gene transcription. SMARCA1/SNF2L is a component of the human NURF (nucleosome remodeling factor) involved in the regulation of genes involved in neuronal development. SMARCA1/SNF2L is also a component of the heterodimeric complex CERF [CECR2(cat eye syndrome chromosome region, candidate 2)-containing remodeling factor]. Alternate names for SMARCA1/SNF2L include SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a1, SNF2, SNF2L, SNF2L1, SNF2LB, sucrose nonfermenting 2-like protein 1, NURF140, and ISWI.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SMARCA1
Entrez
Uniprot