![Immunohistochemistry: SMARCA1 Antibody [NB100-57524] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SMARCA1/SNF2L used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SMARCA1-Antibody-Immunohistochemistry-NB100-57524-img0003.jpg "Immunohistochemistry: SMARCA1 Antibody [NB100-57524] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SMARCA1/SNF2L used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit SMARCA1 Antibody - BSA Free (NB100-57524) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1004 and 1054 of human SMARCA1/SNF2L using the numbering given in entry NP_003060.2 (GeneID 6594). |
| Predicted Species | Mouse (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SMARCA1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is not recommended for blotting crude sources of SMARCA1/SNF2L. NB100-57524 can be used at 1:2,000 to 1:10,000 for blotting enriched sources (e.g. immunoprecipitated and exogenously expressed) of SMARCA1/SNF2L. Immunohistochemistry 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SMARCA1 |
| Entrez |
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| Uniprot |
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![Immunoprecipitation: SMARCA1 Antibody [NB100-57524] - Detection of human SMARCA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMARCA1 antibody NB100-57524 (lot 2) used for IP at 6 ug per reaction. SMARCA1 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated SMARCA1, NB100-57524 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SMARCA1-Antibody-Immunoprecipitation-NB100-57524-img0004.jpg "Immunoprecipitation: SMARCA1 Antibody [NB100-57524] - Detection of human SMARCA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMARCA1 antibody NB100-57524 (lot 2) used for IP at 6 ug per reaction. SMARCA1 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated SMARCA1, NB100-57524 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")