ACF1 Antibody - BSA Free

Analysis in human testis and pancreas tissues using NBP1-90270 antibody. Corresponding BAZ1A RNA-seq data are presented for the same tissues.

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Staining of human tonsil shows strong nuclear positivity in germinal center cells.

Staining of human placenta shows strong nuclear positivity in syncytiotrophoblast.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ACF1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ACF1 Antibody - BSA Free (NBP1-90270) is a polyclonal antibody validated for use in IHC. Anti-ACF1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: RGHRESALKETLLQEKSRICAQLARFSEEKFHFSDKPQPDSKPTYSRGRSSNAYDPSQMCAEKQLELRLRDFLLDIEDRIYQGTLGAIKVTDRHIWRSALESGRYELLSEENKENGIIKTVNEDVEEMEIDEQTK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BAZ1A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  ACF1 Protein (NBP1-90270PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (83%). Reactivity reported in scientific literature (PMID: 24244200)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ACF1 Antibody - BSA Free

• ACF1hWALp1
• ATP-dependent chromatin remodeling protein
• ATP-dependent chromatin-remodeling protein
• ATP-utilizing chromatin assembly and remodeling factor 1
• bromodomain adjacent to zinc finger domain protein 1A
• bromodomain adjacent to zinc finger domain, 1A
• CHRAC subunit ACF1
• hACF1FLJ14383
• WALp1
• WCRF180DKFZp586E0518
• Williams syndrome transcription factor-related chromatin-remodeling factor 180

Background

ACF1/BAZ1A is a subunit of the ACF chromatin-remodeling complex and the ISWI CHRAC remodeling complex. In the ACF complex, ACF1/BAZ1A functions to influence the nucleosome remodeling activity of the SNF2h ATPase. As part of the CHRAC complex, ACF1/BAZ1A may function to target CHRAC to heterochromatin. ACF1 has also been shown to interact with the nuclear receptor corepressor protein (N-CoR) to facilitate the repression of transcription by unliganded nuclear receptors. Alternate names for ACF1/BAZ1A include ATP-utilizing chromatin assembly and remodeling factor1, hACF1, bromodomain adjacent to zinc finger domain protein 1A, Williams syndrome transcription factor-related chromatin-remodeling factor 180, WCRF180, hWALp1, and CHRAC subunit ACF1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACF1 Antibody (NBP1-90270)(1)

Publication using NBP1-90270

• Dowdle JA, Mehta M, Kass EM et al. Mouse BAZ1A (ACF1) Is Dispensable for Double-Strand Break Repair but Is Essential for Averting Improper Gene Expression during Spermatogenesis. PLoS Genet 2013-11-01 [PMID: 24244200]

Bioinformatics

• Gene Symbol: BAZ1A