SMARCA5/SNF2H Antibody

Western Blot: SMARCA5/SNF2H Antibody [NB100-55310] - SNF2H Antibody [NB100-55310] - Detection of Human SNF2h/ISWI on HeLa whole cell lysate using NB100-55310. SNF2h/ISWI was also immunoprecipitated by rabbit anti-SNF2h/ISWI antibodies BL4495 and NB100-55311.

Western Blot: SMARCA5/SNF2H Antibody [NB100-55310] - WB analysis of SNF2H in Mouse Stem Cell lysate. Verified customer review from 1DegreeBio.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

SMARCA5/SNF2H Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human SNF2h/ISWI using the numbering given in entry NP_003592.2 (GeneID 8467).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMARCA5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 122 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  SMARCA5/SNF2H Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SMARCA5/SNF2H Antibody

• EC 3.6.1
• EC 3.6.4.-
• hISWI
• hSNF2HSWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5
• ISWI
• SMARCA5
• SNF2H
• SNF2Hsucrose nonfermenting-like 5
• subfamily a, member 5
• Sucrose nonfermenting protein 2 homolog
• SWI/SNF related, matrix associated, actin dependent regulator of chromatin
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 5
• WCRF135

Background

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SNF2h/ISWI, also known as WCRF135, is the gene product of SMARCA5 that associates with WSTF (Williams Syndrome transcription factor) and several other nuclear proteins to mediate chromatin remodeling. SNF2h is also part of the NoRC (nucleolar remodeling complex).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SMARCA5/SNF2H Antibody (NB100-55310)(7)

Publications using NB100-55310

• Slabber CF, Bachofner M, Speicher T et al. The ubiquitin ligase Uhrf2 is a master regulator of cholesterol biosynthesis and is essential for liver regeneration Science signaling 2023-05-30 [PMID: 37253089] (PLA, Mouse)
  
  Details:
  AML12 cells
• Oppikofer M, Sagolla M, et al. Non-canonical reader modules of BAZ1A promote recovery from DNA damage. Nat Commun 2017-10-11 [PMID: 29021563] (ICC/IF, Human)
• Oppikofer M, Bai T, et al. Expansion of the ISWI chromatin remodeler family with new active complexes. EMBO Rep 2017-10-01 [PMID: 28801535] (WB)
• Turcatel G, Rubin N, El-Hashash A et al. MIR-99a and MIR-99b modulate TGF-beta induced epithelial to mesenchymal plasticity in normal murine mammary gland cells. PLoS One 2012-01-01 [PMID: 22299047]
• Melvin A, Mudie S, Rocha S. The chromatin remodeler ISWI regulates the cellular response to hypoxia: role of FIH. Mol Biol Cell 2011-11-01 [PMID: 21900490]
• Brestovitsky A, Sharf R, Mittelman K et al. The adenovirus E4orf4 protein targets PP2A to the ACF chromatin-remodeling factor and induces cell death through regulation of SNF2h-containing complexes. Nucleic Acids Res 2011-08-01 [PMID: 21546548]
• Bryant KF, Colgrove RC, Knipe DM et al. Cellular SNF2H chromatin-remodeling factor promotes herpes simplex virus 1 immediate-early gene expression and replication. MBio 2011-01-01 [PMID: 21249171]

Review for SMARCA5/SNF2H Antibody (NB100-55310) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 11/06/2013

Summary

• Application: Western Blot
• Sample Tested: Mouse stem cells
• Species: Mouse
• Lot: A1

Bioinformatics

• Gene Symbol: SMARCA5
• Entrez:
  • 8467(Human)
  • 93762(Mouse)
• Uniprot:
  • O60264(Human)