BAF57 Antibody

Western Blot: BAF57 Antibody [NBP1-90012] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunocytochemistry/Immunofluorescence: BAF57 Antibody [NBP1-90012] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012] - Staining in human endometrium and liver tissues using anti-SMARCE1 antibody. Corresponding SMARCE1 RNA-seq data are presented for the same tissues.

Western Blot: BAF57 Antibody [NBP1-90012] - Analysis in human cell line MOLT-4.

Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012] - Staining of human stomach shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012] - Staining of human endometrium shows high expression.

Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012] - Staining of human liver shows low expression as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

BAF57 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: EEREKEAAEQAERSQSSIVPEEEQAANKGEEKKDDENIPMETEETHLEETTESQQNGEEGTSTPEDKESGQEGVDSMAEEGTSDSNTGSESNSATVEEPPTDPIPEDEK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMARCE1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  BAF57 Protein (NBP1-90012PEP)

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for BAF57 Antibody

• BAF57BRG1-associated factor 57
• chromatin remodeling complex BRG1-associated factor 57
• subfamily e, member 1
• SWI/SNF related, matrix associated, actin dependent regulator of chromatin
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily E member 1

Background

BAF57 is encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BAF57 Antibody (NBP1-90012)(2)

Publications using NBP1-90012

• Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261]
• Stadler C, Hjelmare M, Neumann B et al. Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy. J Proteomics 2012-04-03 [PMID: 22361696]

FAQs for BAF57 Antibody (NBP1-90012). (Showing 1 - 1 of 1 FAQs).

1. Can you tell me how baf 57 stains in normal breast tissue? and provide reference/pictures?
   • You can find images using this antibody to stain normal breast tissue here on Human Protein Atlas as this direct antibody was used for those staining images.

Bioinformatics

• Gene Symbol: SMARCE1
• Entrez:
  • 6605(Human)
• Uniprot:
  • Q969G3(Human)