SMARCC1 Antibody

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Western Blot: SMARCC1 Antibody [NBP2-20415] - Wild-type (WT) and SMARCC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody diluted at 1:5000. ...read more
Immunocytochemistry/ Immunofluorescence: SMARCC1 Antibody [NBP2-20415] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMARCC1 protein stained by SMARCC1 antibody (C2C3), C-term diluted at ...read more
Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Paraffin-embedded rat hind brain dilution: 1:500.
Biological Strategies: Chromatin Immunoprecipitation: SMARCC1 Antibody [NBP2-20415] - Cross-linked ChIP was performed with MF-7 chromatin extract treated with B-estradiol (10 nM for 45 min) and 5 ug of either ...read more
Western Blot: SMARCC1 Antibody [NBP2-20415] - Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 C: U87-MG 5% SDS PAGE gel, diluted at 1:5000.
Western Blot: SMARCC1 Antibody [NBP2-20415] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated ...read more
Western Blot: SMARCC1 Antibody [NBP2-20415] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: SMARCC1 Antibody [NBP2-20415] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMARCC1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are ...read more
Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Paraffin-embedded mouse duodenum dilution: 1:50
Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Paraffin-embedded mouse prostate dilution: 1:500.
Immunoprecipitation: SMARCC1 Antibody [NBP2-20415] - SMARCC1 antibody immunoprecipitates SMARCC1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of SMARCC1 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Order Details

SMARCC1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human SMARCC1. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMARCC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:20000
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Application Notes
ChIP assay Assay-dependent dilution
Theoretical MW
123 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
SMARCC1 Knockout HeLa Cell Lysate
Publications
Read Publication using
NBP2-20415 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for SMARCC1 Antibody

  • BAF155SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily C member 1
  • BRG1-associated factor 155
  • chromatin remodeling complex BAF155 subunit
  • CRACC1
  • mammalian chromatin remodeling complex BRG1-associated factor 155
  • Rsc8
  • SRG3
  • subfamily c, member 1
  • SWI/SNF complex 155 kDa subunit
  • SWI/SNF complex subunit SMARCC1
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin
  • SWI3

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for SMARCC1 Antibody (NBP2-20415)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for SMARCC1 Antibody (NBP2-20415) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol SMARCC1
Uniprot