Smad7 Antibody

Images

 
Western Blot: Smad7 Antibody [NBP2-24710] - Effect of mTOR inhibitors on TGF-beta activation of mTOR and Smad pathways. Serum-deprived IPF fibroblasts were treated with or without TGF-beta for 15 minutes in the presence ...read more
Immunohistochemistry-Paraffin: Smad7 Antibody [NBP2-24710] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human liver using Smad7 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 ...read more
Western Blot: Smad7 Antibody [NBP2-24710] - Analysis using SMAD7 antibody. Lysate from HepG2 cells probed with SMAD7 antibody.
Western Blot: Smad7 Antibody [NBP2-24710] - Analysis using the Azide Free version of NBP2-24710. Detection of lysate from A549 cells probed with SMAD7 antibody.

Product Details

Summary
Reactivity Hu, Mu, Sh, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Smad7 Antibody Summary

Immunogen
This antibody was raised against two peptides made from within amino acids 10 and 50 of human SMAD7. This sequence is 100% or highly conserved between human and multiple species.
Specificity
This antibody may cross-react with SMAD6 depending on cell or tissue-specific expression.
Predicted Species
Mouse (92%), Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMAD7
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-5 ug/ml
  • ELISA
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation
Application Notes
Use in ELISA reported in scientific literature (PMID:32108968).
Publications
Read Publications using
NBP2-24710 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    6 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24576683).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Smad7 Antibody

  • CRCS3
  • FLJ16482
  • hSMAD7
  • MAD homolog 7
  • MAD homolog 8
  • MAD, mothers against decapentaplegic homolog 7 (Drosophila)
  • MADH7
  • MADH7mothers against decapentaplegic homolog 7
  • MADH8
  • Mothers against decapentaplegic homolog 8
  • Mothers against decapentaplegic, drosophila, homolog of, 7
  • Mothers against DPP homolog 7
  • Mothers against DPP homolog 8
  • SMAD 7
  • SMAD family member 7MAD (mothers against decapentaplegic, Drosophila) homolog 7
  • SMAD, mothers against DPP homolog 7 (Drosophila)
  • SMAD, mothers against DPP homolog 7
  • Smad7

Background

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature. Human SMAD1 is a 465 amino acid protein; GenBank Accession No. AAP36050.1. Human SMAD2 is a 467 amino acid protein; GenBank Accession No. AAC51918.1 Human SMAD3 is a 425 amino acid protein; GenBank Accession No. NP_005893.1 Human SMAD4 is a 552 amino acid protein GenBank Accession No. NP_005350.1. Human SMAD5 is a 465 amino acid protein; GenBank Accession No. AAC50791.1. Human SMAD6 is a 496 amino acid protein; GenBank Accession No. AAH12986.1 Human SMAD7 is a 426 amino acid protein; GenBank Accession No. AAB81354.1 Mouse SMAD8 is a 430 amino acid protein; GenBank Accession No. AAN85445.1 Human SMAD9 is a 430 amino acid protein; GenBank Accession No. NP_005896.1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Smad7 Antibody (NBP2-24710)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Sheep.

We have publications tested in 3 applications: ELISA, IHC, WB.


Filter By Application
ELISA
(1)
IHC
(1)
WB
(6)
All Applications
Filter By Species
Human
(5)
Mouse
(2)
Sheep
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NBP2-24710 Applications Species
Shrestha R, Mohankumar K, Safe S Bis-indole derived nuclear receptor 4A1 (NR4A1) antagonists inhibit TGF beta-induced invasion of embryonal rhabdomyosarcoma cells American journal of cancer research Aug 1 2020 [PMID: 32905449] (WB, Human) WB Human
Yang P, Dong X, Zhang Y MicroRNA profiles in plasma samples from young metabolically healthy obese patients and miRNA-21 are associated with diastolic dysfunction via TGF- beta 1/Smad pathway J. Clin. Lab. Anal. Feb 28 2020 [PMID: 32108968] (ELISA, Human) ELISA Human
Tang Y, Reissig S, Glasmacher E et al. Alternative Splice Forms of CYLD Mediate Ubiquitination of SMAD7 to Prevent TGFB Signaling and Promote Colitis Gastroenterology Feb 1 2019 [PMID: 30315770] (WB, Mouse) WB Mouse
Singha PK, Pandeswara S, Geng H et al. TGF-b induced TMEPAI/PMEPA1 inhibits canonical Smad signaling through R-Smad sequestration and promotes non-canonical PI3K/Akt signaling by reducing PTEN in triple negative breast cancer. Genes Cancer. 2014 Sep 01 [PMID: 25352949] (WB, Human)

Details:
Smad7 antibody used for WB on MDA-MB-231 and HCC1937 cell lines expressing control shRNA or TMEPAI shRNA (Fig 3).
WB Human
Chang W, Wei K, Ho L et al. A Critical Role for the mTORC2 Pathway in Lung Fibrosis PLoS ONE 2014 Aug 28 [PMID: 25162417] (WB, Human)

Details:
SMAD7 antibody used for WB on lysates of fibroblasts (isolated from the surgical lung explants 0 Idiopathic Pulmonary Fibrosis /IPF) stimulated with TGF beta and treated or not with inhibitors MLN0128 (0.2 uM), PP242 (2 uM), or rapamycin (0.02 uM).
WB Human
Kayalar O, Oztay F. Retinoic acid induced repair in the lung of adult hyperoxic mice, reducing transforming growth factor-B1 (TGF-B1) mediated abnormal alterations. Acta Histochem. [PMID: 24576683] (WB, Mouse)

Details:
Citation using the Azide Free form of this antibody.
WB Mouse
L0nn P, Vanlandewijck M, Raja E et al. Transcriptional induction of salt-inducible kinase 1 by transforming growth factor B leads to negative regulation of type I receptor signaling in cooperation with the Smurf2 ubiquitin ligase. J Biol Chem. 2012 Apr 13 [PMID: 22378783]
Hogg K, Etherington SL, Young JM et al. Inhibitor of differentiation (Id) genes are expressed in the steroidogenic cells of the ovine ovary and are differentially regulated by members of the transforming growth factor-beta family. Endocrinology. 2010 Mar [PMID: 20032053] (IHC, Sheep)

Details:
Antibodies cited (sheep ovary): 1. SMAD6 (IMG-555): IHC (Bouin's-fixed, paraffin-embedded), Fig 3G,H; IF, Fig 4B. 2. SMAD7 (IMG-531): IHC (Bouin's-fixed, paraffin-embedded), Fig 3J,K. Note: See Table 1 for antibody dilutions used.
IHC Sheep
Wang Q, Wang Y, Minto AW et al. MicroRNA-377 is up-regulated and can lead to increased fibronectin production in diabetic nephropathy. FASEB J. 2008 Dec [PMID: 18716028] (WB, Human)

Details:
WB (human mesangial cells), Fig. 3A.
WB Human
Roman-Blas JA, Stokes DG, Jimenez SA. Modulation of TGF-beta signaling by proinflammatory cytokines in articular chondrocytes. Osteoarthritis Cartilage. 2007 Dec [PMID: 17604656]
Show All 12 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-24710AF350
Alexa Fluor 405 NBP2-24710AF405
Alexa Fluor 488 NBP2-24710AF488
Alexa Fluor 532 NBP2-24710AF532
Alexa Fluor 594 NBP2-24710AF594
Alexa Fluor 647 NBP2-24710AF647
Alexa Fluor 700 NBP2-24710AF700
Alexa Fluor 750 NBP2-24710AF750
Biotin NBP2-24710B
DyLight 350 NBP2-24710UV
DyLight 405 NBP2-24710V
DyLight 488 NBP2-24710G
DyLight 550 NBP2-24710R
DyLight 594 NBP2-24710DL594
DyLight 650 NBP2-24710C
DyLight 680 NBP2-24710FR
DyLight 755 NBP2-24710IR
FITC NBP2-24710F
HRP NBP2-24710H
Janelia Fluor 549 NBP2-24710JF549
Janelia Fluor 646 NBP2-24710JF646

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Bioinformatics

Gene Symbol SMAD7
Uniprot