Smad7 Antibody

Western Blot: Smad7 Antibody [NBP2-24710] - Analysis using the Azide Free version of NBP2-24710. Detection of lysate from A549 cells probed with SMAD7 antibody.

Immunohistochemistry-Paraffin: Smad7 Antibody [NBP2-24710] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human liver using Smad7 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Smad7 was observed. Staining was performed by Histowiz.

Western Blot: Smad7 Antibody [NBP2-24710] - Analysis using SMAD7 antibody. Lysate from HepG2 cells probed with SMAD7 antibody.

• Reactivity: Hu, Mu, Sh, Mu, Rt
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Smad7 Antibody Summary

• Immunogen: This antibody was raised against two peptides made from within amino acids 10 and 50 of human SMAD7. This sequence is 100% or highly conserved between human and multiple species.
• Specificity: This antibody may cross-react with SMAD6 depending on cell or tissue-specific expression.
• Predicted Species: Mouse (92%), Rat (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMAD7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2-5 ug/ml
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24576683).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Smad7 Antibody

• CRCS3
• FLJ16482
• hSMAD7
• MAD homolog 7
• MAD homolog 8
• MAD, mothers against decapentaplegic homolog 7 (Drosophila)
• MADH7
• MADH7mothers against decapentaplegic homolog 7
• MADH8
• Mothers against decapentaplegic homolog 8
• Mothers against decapentaplegic, drosophila, homolog of, 7
• Mothers against DPP homolog 7
• Mothers against DPP homolog 8
• SMAD 7
• SMAD family member 7MAD (mothers against decapentaplegic, Drosophila) homolog 7
• SMAD, mothers against DPP homolog 7 (Drosophila)
• SMAD, mothers against DPP homolog 7
• Smad7

Background

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature. Human SMAD1 is a 465 amino acid protein; GenBank Accession No. AAP36050.1. Human SMAD2 is a 467 amino acid protein; GenBank Accession No. AAC51918.1 Human SMAD3 is a 425 amino acid protein; GenBank Accession No. NP_005893.1 Human SMAD4 is a 552 amino acid protein GenBank Accession No. NP_005350.1. Human SMAD5 is a 465 amino acid protein; GenBank Accession No. AAC50791.1. Human SMAD6 is a 496 amino acid protein; GenBank Accession No. AAH12986.1 Human SMAD7 is a 426 amino acid protein; GenBank Accession No. AAB81354.1 Mouse SMAD8 is a 430 amino acid protein; GenBank Accession No. AAN85445.1 Human SMAD9 is a 430 amino acid protein; GenBank Accession No. NP_005896.1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Smad7 Antibody (NBP2-24710)(10)

Publications using NBP2-24710

• Tang Y, Reissig S, Glasmacher E et al. Alternative Splice Forms of CYLD Mediate Ubiquitination of SMAD7 to Prevent TGFB Signaling and Promote Colitis Gastroenterology Feb 1 2019 [PMID: 30315770] (WB, Mouse)
• Singha PK, Pandeswara S, Geng H et al. TGF-b induced TMEPAI/PMEPA1 inhibits canonical Smad signaling through R-Smad sequestration and promotes non-canonical PI3K/Akt signaling by reducing PTEN in triple negative breast cancer. Genes Cancer. 2014 Sep 01 [PMID: 25352949] (WB, Human)
  
  Details:
  Smad7 antibody used for WB on MDA-MB-231 and HCC1937 cell lines expressing control shRNA or TMEPAI shRNA (Fig 3).
• Chang W, Wei K, Ho L et al. A Critical Role for the mTORC2 Pathway in Lung Fibrosis PLoS ONE 2014 Aug 28 [PMID: 25162417] (WB, Human)
  
  Details:
  SMAD7 antibody used for WB on lysates of fibroblasts (isolated from the surgical lung explants 0 Idiopathic Pulmonary Fibrosis /IPF) stimulated with TGF beta and treated or not with inhibitors MLN0128 (0.2 uM), PP242 (2 uM), or rapamycin (0.02 uM).
• Kayalar O, Oztay F. Retinoic acid induced repair in the lung of adult hyperoxic mice, reducing transforming growth factor-B1 (TGF-B1) mediated abnormal alterations. Acta Histochem. [PMID: 24576683] (WB, Mouse)
  
  Details:
  Citation using the Azide Free form of this antibody.
• L0nn P, Vanlandewijck M, Raja E et al. Transcriptional induction of salt-inducible kinase 1 by transforming growth factor B leads to negative regulation of type I receptor signaling in cooperation with the Smurf2 ubiquitin ligase. J Biol Chem. 2012 Apr 13 [PMID: 22378783]
• Hogg K, Etherington SL, Young JM et al. Inhibitor of differentiation (Id) genes are expressed in the steroidogenic cells of the ovine ovary and are differentially regulated by members of the transforming growth factor-beta family. Endocrinology. 2010 Mar [PMID: 20032053] (IHC, Sheep)
  
  Details:
  Antibodies cited (sheep ovary): 1. SMAD6 (IMG-555): IHC (Bouin's-fixed, paraffin-embedded), Fig 3G,H; IF, Fig 4B. 2. SMAD7 (IMG-531): IHC (Bouin's-fixed, paraffin-embedded), Fig 3J,K. Note: See Table 1 for antibody dilutions used.
• Wang Q, Wang Y, Minto AW et al. MicroRNA-377 is up-regulated and can lead to increased fibronectin production in diabetic nephropathy. FASEB J. 2008 Dec [PMID: 18716028] (WB, Human)
  
  Details:
  WB (human mesangial cells), Fig. 3A.
• Roman-Blas JA, Stokes DG, Jimenez SA. Modulation of TGF-beta signaling by proinflammatory cytokines in articular chondrocytes. Osteoarthritis Cartilage. 2007 Dec [PMID: 17604656]
• Kleiter Ingo, Pedre Xiomara, Mueller Andre M et al. Inhibition of Smad7, a negative regulator of TGF-beta signaling, suppresses autoimmune encephalomyelitis. J Neuroimmunol. 2007 Jul [PMID: 17553571]
• Kidd M, Modlin IM, Pfragner R et al. Small bowel carcinoid (enterochromaffin cell) neoplasia exhibits transforming growth factor-beta1-mediated regulatory abnormalities including up-regulation of C-Myc and MTA1. Cancer. 2007 Jun 15 [PMID: 17469181]
  
  Details:
  WB: SMAD4 (IMG-565) and B-actin (IMG-531). Neoplastic kRJ-I enterochromaffin (EC) cells stimulated with growth factor-B1 (TGFB1), Fig 4.

Bioinformatics

• Gene Symbol: SMAD7
• Uniprot:
  • NP_005895(Human)