Western Blot: Smad4 Antibody [NBP2-24951] - SMAD4 Antibody [NBP2-24951] - Analysis using SMAD4 antibody. Lysate from A549 cells probed with SMAD4 antibody at 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: Smad4 Antibody [NBP2-24951] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Immunohistochemistry-Paraffin: Smad4 Antibody [NBP2-24951] - Analysis of a FFPE tissue section of human colon using 1:200 dilution of SMAD4 antibody. The staining was developed using HRP labeled anti-rabbit secondary ...read more
This antibody was developed against synthetic peptides corresponding to amino acids 186-223 and amino acids 509-544 of human SMAD4 (GenBank Accession No. NP_005350.1). These peptide sequences are specific to SMAD4, and have high homology to SMAD4 in multiple species.
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Bovine reactivity reported from a verified customer review.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Alternate Names for Smad4 Antibody
DPC4SMAD, mothers against DPP homolog 4 (Drosophila)
MAD homolog 4
MADH4mothers against decapentaplegic homolog 4
mothers against decapentaplegic homolog 4
mothers against decapentaplegic, Drosophila, homolog of, 4
SMAD family member 4MAD, mothers against decapentaplegic homolog 4 (Drosophila)
SMAD, mothers against DPP homolog 4
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFbeta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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