Smad4 Antibody

Western Blot: Smad4 Antibody [NBP2-24951] - SMAD4 Antibody [NBP2-24951] - Analysis using SMAD4 antibody. Lysate from A549 cells probed with SMAD4 antibody at 2 ug/mL.

Immunocytochemistry/Immunofluorescence: Smad4 Antibody [NBP2-24951] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-SMAD4 Antibody NBP2-24951 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: Smad4 Antibody [NBP2-24951] - Analysis of a FFPE tissue section of human colon using 1:200 dilution of SMAD4 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Apical cytoplasmic staining was observed in both glandular and epithelial cells.

• Reactivity: Hu, Mu, Rt, Bv, Pm
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Smad4 Antibody Summary

• Immunogen: This antibody was developed against synthetic peptides corresponding to amino acids 186-223 and amino acids 509-544 of human SMAD4 (GenBank Accession No. NP_005350.1). These peptide sequences are specific to SMAD4, and have high homology to SMAD4 in multiple species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMAD4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1 - 5 ug/mL
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1 - 3 ug/mL

Reactivity Notes

Bovine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Smad4 Antibody

• DPC4
• DPC4SMAD, mothers against DPP homolog 4 (Drosophila)
• hSMAD4
• JIP
• MAD homolog 4
• MADH4
• MADH4mothers against decapentaplegic homolog 4
• mothers against decapentaplegic homolog 4
• mothers against decapentaplegic, Drosophila, homolog of, 4
• SMAD 4
• SMAD family member 4MAD, mothers against decapentaplegic homolog 4 (Drosophila)
• SMAD, mothers against DPP homolog 4
• Smad4

Background

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFbeta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Smad4 Antibody (NBP2-24951)(2)

Publications using NBP2-24951

• Tumelty KE, Higginson-Scott N, Fan X et al. Identification of direct negative crosstalk between the SLIT2 and Bone Morphogenetic Protein-Gremlin signaling pathways J. Biol. Chem. 2018-01-09 [PMID: 29317497] (WB, Human)
• Kidd M, Modlin IM, Pfragner R et al. Small bowel carcinoid (enterochromaffin cell) neoplasia exhibits transforming growth factor-beta1-mediated regulatory abnormalities including up-regulation of C-Myc and MTA1. Cancer. 2007-06-15 [PMID: 17469181] (WB)
  
  Details:
  WB: SMAD4 (IMG-565) and B-actin (IMG-531). Neoplastic kRJ-I enterochromaffin (EC) cells stimulated with growth factor-B1 (TGFB1), Fig 4.

Reviews for Smad4 Antibody (NBP2-24951) (3)

Average Rating: 3.7

(Based on 3 reviews)

We have 3 reviews tested in 3 species: Human, Mouse, Bovine.

reviewed by: Ajay Ashok WB Human 05/26/2021

Summary

• Application: Western Blot
• Sample Tested: eye
• Species: Human
• Lot: AB102003A-1

Comments

• Comments: used at 1:100 overnight

reviewed by: Ajay Ashok WB Mouse 05/26/2021

Summary

• Application: Western Blot
• Sample Tested: mouse eye
• Species: Mouse
• Lot: AB102003A-1

Comments

• Comments: used at 1:100 overnight

reviewed by: Ajay Ashok WB Bovine 05/26/2021

Summary

• Application: Western Blot
• Sample Tested: eye
• Species: Bovine
• Lot: AB102003A-1

Comments

• Comments: used at 1:100 overnight

Bioinformatics

• Gene Symbol: SMAD4
• Uniprot:
  • Q13485()