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Smad4 Antibody

Images

 
Western Blot: Smad4 Antibody [NBP2-24951] - SMAD4 Antibody [NBP2-24951] - Analysis using SMAD4 antibody. Lysate from A549 cells probed with SMAD4 antibody at 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: Smad4 Antibody [NBP2-24951] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Immunohistochemistry-Paraffin: Smad4 Antibody [NBP2-24951] - Analysis of a FFPE tissue section of human colon using 1:200 dilution of SMAD4 antibody. The staining was developed using HRP labeled anti-rabbit secondary ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Smad4 Antibody Summary

Immunogen
This antibody was developed against synthetic peptides corresponding to amino acids 186-223 and amino acids 509-544 of human SMAD4 (GenBank Accession No. NP_005350.1). These peptide sequences are specific to SMAD4, and have high homology to SMAD4 in multiple species.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMAD4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1 - 5 ug/mL
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1 - 3 ug/mL
Reviewed Applications
Read 3 Reviews rated 3.7
using
NBP2-24951 in the following applications:

Publications
Read Publications using
NBP2-24951 in the following applications:

  • WB
    2 publications

Reactivity Notes

Bovine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Smad4 Antibody

  • DPC4
  • DPC4SMAD, mothers against DPP homolog 4 (Drosophila)
  • hSMAD4
  • JIP
  • MAD homolog 4
  • MADH4
  • MADH4mothers against decapentaplegic homolog 4
  • mothers against decapentaplegic homolog 4
  • mothers against decapentaplegic, Drosophila, homolog of, 4
  • SMAD 4
  • SMAD family member 4MAD, mothers against decapentaplegic homolog 4 (Drosophila)
  • SMAD, mothers against DPP homolog 4
  • Smad4

Background

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFbeta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Smad4 Antibody (NBP2-24951)(2)

Reviews for Smad4 Antibody (NBP2-24951) (3) 3.73

Average Rating: 3.7
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Human, Mouse, Bovine.

Reviews using NBP2-24951:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Bovine
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Smad4 NBP2-24951
Enlarge
3
reviewed by:
Verified Customer
WB Human 05/26/2021
View

Summary

ApplicationWestern Blot
Sample Testedeye
SpeciesHuman
LotAB102003A-1

Comments

Commentsused at 1:100 overnight
Western Blot Smad4 NBP2-24951
Enlarge
4
reviewed by:
Verified Customer
WB Mouse 05/26/2021
View

Summary

ApplicationWestern Blot
Sample Testedmouse eye
SpeciesMouse
LotAB102003A-1

Comments

Commentsused at 1:100 overnight
Western Blot Smad4 NBP2-24951
Enlarge
4
reviewed by:
Verified Customer
WB Bovine 05/26/2021
View

Summary

ApplicationWestern Blot
Sample Testedeye
SpeciesBovine
LotAB102003A-1

Comments

Commentsused at 1:100 overnight

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Smad4 Antibody (NBP2-24951) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Research Areas for Smad4 Antibody (NBP2-24951)

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Blogs on Smad4

There are no specific blogs for Smad4, but you can read our latest blog posts.
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Contact Information

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Calculators

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Recent Reviews

3.7
5
0
4
2
3
1
2
0
1
0

Verified Customer
05/26/2021
Application: WB
Species: Human

Verified Customer
05/26/2021
Application: WB
Species: Mouse

Verified Customer
05/26/2021
Application: WB
Species: Bovine

Bioinformatics

Gene Symbol SMAD4
Uniprot