| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2097-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24951SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC2097P | |||
| Carrier Free | MAB2097-SP | |||
| Carrier Free | IC2097T-100UG | |||
| Carrier Free | IC2097S-100UG | |||
| Carrier Free | IC2097R-100UG | |||
| Carrier Free | IC2097N-100UG | |||
| Carrier Free | IC2097G-100UG | |||
| Carrier Free | IC2097V-100UG | |||
| Carrier Free | IC2097U-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43874 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB20971-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14116 | |||
![Western Blot: Smad4 Antibody [NBP2-24951] - SMAD4 Antibody [NBP2-24951] - Analysis using SMAD4 antibody. Lysate from A549 cells probed with SMAD4 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/SMAD4-Antibody-Western-Blot-NBP2-24951-img0001.jpg "Western Blot: Smad4 Antibody [NBP2-24951] - SMAD4 Antibody [NBP2-24951] - Analysis using SMAD4 antibody. Lysate from A549 cells probed with SMAD4 antibody at 2 ug/mL.")
![Immunocytochemistry/Immunofluorescence: Smad4 Antibody [NBP2-24951] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-SMAD4 Antibody NBP2-24951 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Smad4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24951-img0003.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody [NBP2-24951] - Analysis of a FFPE tissue section of human colon using 1:200 dilution of SMAD4 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Apical cytoplasmic staining was observed in both glandular and epithelial cells.](http://images.novusbio.com/fullsize/Smad4-Antibody-Immunohistochemistry-Paraffin-NBP2-24951-img0002.jpg)
![Western Blot: Smad4 Antibody (1H7D3) [NBP3-15673] - Western blot analysis of extracts from normal (control) and Smad4 knockout (KO) 293T cells, using Smad4 antibody (NBP3-15673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Smad4-Antibody-1H7D3-Knockout-Validated-NBP3-15673-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Smad4 Antibody (1H7D3) [NBP3-15673] - Western blot analysis of extracts from normal (control) and Smad4 knockout (KO) 293T cells, using Smad4 antibody (NBP3-15673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Smad4 Antibody (1H7D3) [NBP3-15673] -Analysis of various lysates using [KO Validated] Smad4 Rabbit mAb at 1:1000 dilution incubated overnight at 4c.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-15673_rabbit-smad4-mab-6b4d6-western-blot-3012025141330.png)
![Western Blot: Smad4 Antibody (6B4D6) [Smad4] - Western blot analysis of various lysates using [KO Validated] Smad4 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-15673_rabbit-smad4-mab-6b4d6-52202517361256.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (1H7D3) [NBP3-15673] - Analysis of paraffin-embedded Human placenta tissue using [KO Validated] Smad4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15673_rabbit-smad4-mab-6b4d6-immunohistochemistry-paraffin-3012025141623..png)
![Immunohistochemistry: Smad4 Antibody (6B4D6) [Smad4] - Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KO Validated] Smad4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15673_rabbit-smad4-mab-6b4d6-52202517352750.jpg)
![Immunohistochemistry: Smad4 Antibody (6B4D6) [Smad4] - Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KO Validated] Smad4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15673_rabbit-smad4-mab-6b4d6-52202517361235.jpg)
![Chromatin Immunoprecipitation: Smad4 Antibody (6B4D6) [Smad4] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using [KO Validated] Smad4 Rabbit mAb and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15673_rabbit-smad4-mab-6b4d6-52202517344278.jpg)
![Immunohistochemistry: Smad4 Antibody (6B4D6) [Smad4] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KO Validated] Smad4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15673_rabbit-smad4-mab-6b4d6-52202517361264.jpg)
![Immunohistochemistry: Smad4 Antibody (6B4D6) [Smad4] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using [KO Validated] Smad4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15673_rabbit-smad4-mab-6b4d6-52202517363720.jpg)
![Western Blot: Smad4 Antibody (CL9774) [NBP3-05507] - Analysis in human cell line SCLC-21H.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Western-Blot-NBP3-05507-img0006.jpg "Western Blot: Smad4 Antibody (CL9774) [NBP3-05507] - Analysis in human cell line SCLC-21H.")
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human testis shows moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0005.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human placenta shows moderate nuclear and cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0001.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human prostate shows moderate nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0002.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0003.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human stomach cancer shows strong cytoplasmic and nuclear positivity.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0004.jpg)
![Western Blot: Smad4 Antibody (4G1C6) [NBP1-51646] - Analysis using SMAD4 mouse mAb against A431 (1), SK-N-SH (2), K562 (3), HepG2 (4) and HUVE12 (5) cell lysate.](http://images.novusbio.com/fullsize/Smad4-Antibody-4G1C6-Western-Blot-NBP1-51646-img0015.jpg "Western Blot: Smad4 Antibody (4G1C6) [NBP1-51646] - Analysis using SMAD4 mouse mAb against A431 (1), SK-N-SH (2), K562 (3), HepG2 (4) and HUVE12 (5) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Smad4 Antibody (4G1C6) [NBP1-51646] - Analysis of NIH/3T3 cells using SMAD4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Smad4-Antibody-4G1C6-Immunocytochemistry-NBP1-51646-img0012.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (4G1C6) [NBP1-51646] - Analysis of lung cancer tissues using SMAD4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Smad4-Antibody-4G1C6-Immunohistochemistry-Paraffin-NBP1-51646-img0014.jpg)
![Flow Cytometry: Smad4 Antibody (4G1C6) [NBP1-51646] - Analysis of K562 cells using SMAD4 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Smad4-Antibody-4G1C6-Flow-Cytometry-NBP1-51646-img0009.jpg)
![Western Blot: Smad4 Antibody (PD01-37) [NBP3-32956] - Western blot analysis of Smad4 on different lysates with Rabbit anti-Smad4 antibody (NBP3-32956) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Jurkat cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: Ramos cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32956) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32956_rabbit-smad4-mab-pd01-37-288202412461648.jpg "Western Blot: Smad4 Antibody (PD01-37) [NBP3-32956] - Western blot analysis of Smad4 on different lysates with Rabbit anti-Smad4 antibody (NBP3-32956) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Jurkat cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: Ramos cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32956) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Smad4 Antibody (PD01-37) [NBP3-32956] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-Smad4 antibody (NBP3-32956) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32956) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32956_rabbit-smad4-mab-pd01-37-298202414304112.jpg)
![Immunocytochemistry/ Immunofluorescence: Smad4 Antibody (PD01-37) [NBP3-32956] - Immunocytochemistry analysis of PANC-1 cells labeling Smad4 with Rabbit anti-Smad4 antibody (NBP3-32956) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Smad4 antibody (NBP3-32956) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32956_rabbit-smad4-mab-pd01-37-29202410234283.jpg)
![Immunoprecipitation: Smad4 Antibody [NBP3-14714] - : Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from 293T cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-SMAD4recombinant monoclonal antibody used for IP at 6 ul per reaction. SMAD4 was alsoimmunoprecipitated by rabbit anti-SMAD4 antibody. For blotting immunoprecipitated SMAD4, was used at 1:1000. Chemiluminescence withan exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Smad4-Antibody-Immunoprecipitation-NBP3-14714-img0001.jpg "Immunoprecipitation: Smad4 Antibody [NBP3-14714] - : Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from 293T cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-SMAD4recombinant monoclonal antibody used for IP at 6 ul per reaction. SMAD4 was alsoimmunoprecipitated by rabbit anti-SMAD4 antibody. For blotting immunoprecipitated SMAD4, was used at 1:1000. Chemiluminescence withan exposure time of 3 seconds.")
![Western Blot: Smad4 Antibody (RM277) [NBP2-61587] - Western Blot of Jurkat and IMR32 cells lysates using NBP2-61587.](http://images.novusbio.com/fullsize/Smad4-Antibody-RM277-Western-Blot-NBP2-61587-img0003.jpg "Western Blot: Smad4 Antibody (RM277) [NBP2-61587] - Western Blot of Jurkat and IMR32 cells lysates using NBP2-61587.")
![Immunohistochemistry-Paraffin: Smad4 Antibody (RM277) [NBP2-61587] - Immunohistochemical staining of formalin fixed and paraffin embedded human colon cancer tissue sections using NBP2-61587 at a 1:5000 dilution.](http://images.novusbio.com/fullsize/nbp2-61587_rabbit-monoclonal-smad4-antibody-rm277-immunohistochemistry-paraffin-189202523521120.png)
![Immunohistochemistry-Paraffin: Smad4 Antibody (RM277) [NBP2-61587] - Immunohistochemical staining of formalin fixed and paraffin embedded Human breast cancer tissue sections using NBP2-61587.](http://images.novusbio.com/fullsize/nbp2-61587_rabbit-monoclonal-smad4-antibody-rm277-immunohistochemistry-paraffin-18920252338176.png)
![Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD/6309R) [NBP3-13963] - Immunohistochemical stain using Smad4 antibody (SMAD/6309R) showing loss of SMAD4 expression in pancreatic ductal adenocarcinoma. Note adjacent benign ductal epithelium and background stroma as internal control showing positive / retained SMAD4 staining.](http://images.novusbio.com/fullsize/Smad4-Antibody-SMAD-6309R-Immunohistochemistry-Paraffin-NBP3-13963-img0001.jpg "Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD/6309R) [NBP3-13963] - Immunohistochemical stain using Smad4 antibody (SMAD/6309R) showing loss of SMAD4 expression in pancreatic ductal adenocarcinoma. Note adjacent benign ductal epithelium and background stroma as internal control showing positive / retained SMAD4 staining.")
![Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD/6309R) [NBP3-13963] - Formalin-fixed, paraffin-embedded human small intestine stained with Smad4 antibody (SMAD/6309R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Smad4-Antibody-SMAD-6309R-Immunohistochemistry-Paraffin-NBP3-13963-img0002.jpg)
