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HAF019). A specific band was detected for Smad4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
219-IL) overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Smad4/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human Smad4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2097) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thep21promoter was detected by standard PCR." class="big_lightbox" target="_blank">
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFbeta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members.
![Western Blot Smad4 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Smad4_AF2097_Western_Blot_17168.jpg)
![Chromatin Immunoprecipitation (ChIP) Smad4 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Smad4_AF2097_Chromatin_Immunoprecipitation_11225.jpg)
![Immunocytochemistry Smad4 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Smad4_AF2097_Immunocytochemistry__Immunofluorescence_17807.jpg)
![Western Blot Smad4 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2097_human-smad4-affinity-purified-polyclonal-ab-41202412401211.jpg)
![Western Blot Smad4 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2097_human-smad4-affinity-purified-polyclonal-ab-41202412394910.jpg)
