Human Smad1 ELISA Kit (Colorimetric)

ELISA: Human Smad1 ELISA Kit (Colorimetric) [NBP2-76618] - Samples were spiked with high concentrations of Human Smad1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Smad1 ELISA Kit (Colorimetric) [NBP2-76618] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.781 - 50ng/mL
• Sensitivity: 0.469ng/mL

Human Smad1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human Smad1/MADH1 in samples. No significant cross-reactivity or interference between Human Smad1/MADH1 and analogues was observed.
• Standard Curve Range: 0.781 - 50ng/mL
• Sensitivity: 0.469ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.07
• Intra-Assay: CV% < 4.2
• Spike Recovery: 85-105%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SMAD1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Smad1 ELISA Kit (Colorimetric)

• BSP1
• BSP-1
• hSMAD1
• JV4-1SMAD, mothers against DPP homolog 1 (Drosophila)
• MAD homolog 1
• MADH1JV41
• MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila)
• Mad-related protein 1
• mothers against decapentaplegic homolog 1
• Mothers against DPP homolog 1
• SMAD family member 1SMAD 1
• SMAD, mothers against DPP homolog 1
• Smad1
• TGF-beta signaling protein 1
• transforming growth factor-beta signaling protein 1

Background

Smad1 is encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Smad1 ELISA Kit (NBP2-76618). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: SMAD1