This antibody was developed against two synthetic peptides corresponding to amino acids 18-32 (LGWKQGDEEEKWAEK) and amino acids 315-330 (SNVNRNSTIENTRRHI) of human SMAD1. GenBank Accession No. AAP36050.1.
In A549, a 50 kDa band is observed. The two synthetic peptide immunogens (LGWKQGDEEEKWAEK) and (SNVNRNSTIENTRRHI) used to make this antibody are 100% conserved between SMAD1/5/8/9. Therefore, the antibody recognizes SMADs 1, 5, 8, and 9. The antibody may cross-react with SMAD2 or SMAD3. The actual SMADs detected with this antibody may vary according to cell or tissue-type specific expression.
Protein G purified
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The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
52 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NB100-56443 in the following applications:
Predicted Reactivity: Most mammals, Chimpanzee, Porcine, Zebrafish, Xenopus, Chicken, invertebrates.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 0.05% BSA
0.05% Sodium Azide
Protein G purified
Alternate Names for SMAD1/5/8/9 Antibody
JV4-1SMAD, mothers against DPP homolog 1 (Drosophila)
MAD homolog 1
MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila)
Mad-related protein 1
mothers against decapentaplegic homolog 1
Mothers against DPP homolog 1
SMAD family member 1SMAD 1
SMAD, mothers against DPP homolog 1
TGF-beta signaling protein 1
transforming growth factor-beta signaling protein 1
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.