SMAD1/5/8/9 Antibody

Western Blot: SMAD1/5/8/9 Antibody [NB100-56443] - Lysate fromA549 cells probed with SMAD antibody at 2 ug/mL.

Product Discontinued

SMAD1/5/8/9 Antibody Summary

• Immunogen: This antibody was developed against two synthetic peptides corresponding to amino acids 18-32 (LGWKQGDEEEKWAEK) and amino acids 315-330 (SNVNRNSTIENTRRHI) of human SMAD1. GenBank Accession No. AAP36050.1.
• Specificity: In A549, a 50 kDa band is observed. The two synthetic peptide immunogens (LGWKQGDEEEKWAEK) and (SNVNRNSTIENTRRHI) used to make this antibody are 100% conserved between SMAD1/5/8/9. Therefore, the antibody recognizes SMADs 1, 5, 8, and 9. The antibody may cross-react with SMAD2 or SMAD3. The actual SMADs detected with this antibody may vary according to cell or tissue-type specific expression.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMAD1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 - 3 ug/mL
• Application Notes: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted Reactivity: Most mammals, Chimpanzee, Porcine, Zebrafish, Xenopus, Chicken, invertebrates.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for SMAD1/5/8/9 Antibody

• BSP1
• BSP-1
• hSMAD1
• JV4-1SMAD, mothers against DPP homolog 1 (Drosophila)
• MAD homolog 1
• MADH1JV41
• MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila)
• Mad-related protein 1
• mothers against decapentaplegic homolog 1
• Mothers against DPP homolog 1
• SMAD family member 1SMAD 1
• SMAD, mothers against DPP homolog 1
• Smad1
• TGF-beta signaling protein 1
• transforming growth factor-beta signaling protein 1

Background

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SMAD1/5/8/9 Antibody (NB100-56443)(2)

Publications using NB100-56443

• Hong D, Liu T, Huang W et al. Gremlin1 Delivered by Mesenchymal Stromal Cells Promoted Epithelial-Mesenchymal Transition in Human Esophageal Squamous Cell Carcinoma. Cell. Physiol. Biochem. 2018-06-28 [PMID: 29953975] (WB, Human)
• An Q, Gou B, Ma S et al. Icariin induces MC3T3-E1 cell proliferation and differentiation via the BMP-2/Smads/Runx2 signal pathway. bioRxiv 2018-08-31 (WB, Mouse)

Bioinformatics

• Gene Symbol: SMAD1
• Entrez:
  • 4086(Human)
  • 17125(Mouse)
  • 25671(Rat)
• Uniprot:
  • Q15797()