SMAC/Diablo Antibody


Western Blot: SMAC/Diablo Antibody [NBP1-76772] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: SMAC/Diablo NBP1-76772 (1 ug/mL), 1h incubation at RT in 5% more
Immunocytochemistry/ Immunofluorescence: SMAC/Diablo Antibody [NBP1-76772] - Immunofluorescence of Smac in Mouse Spleen cells with Smac antibody at 10 ug/mL.
Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NBP1-76772] - Immunohistochemical staining of mouse spleen tissue using Smac antibody at 2 ug/ml.
Western Blot: SMAC/Diablo Antibody [NBP1-76772] - Analysis of Smac in mouse heart tissue lysate in the absence (A) or presence (B) of blocking peptide and in rat heart tissue lysate with Smac antibody at 1 ug /ml.
Independent Antibodies: Western Blot: SMAC/Diablo Antibody [NBP1-76772] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Validated by:

Independent Antibodies


Order Details

SMAC/Diablo Antibody Summary

Antibody was raised against a 16 amino acid peptide near the carboxy terminus of murine Smac. The immunogen is located within the last 50 amino acids of Smac. Amino Acid Squence: SDEGADQEEEAYLRED
Peptide affinity purified
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  • Western Blot 1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Immunoprecipitation
Application Notes
Smac antibody can be used for detection of Smac by Western blot at 0.5 to 1 mg/ml. A 25 kDa band can be detected.
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
SMAC/Diablo Peptide (NBP1-76772PEP)
Read Publications using
NBP1-76772 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Peptide affinity purified

Alternate Names for SMAC/Diablo Antibody

  • 0610041G12Rik
  • Diablo
  • diablo, IAP-binding mitochondrial protein
  • Direct IAP-binding protein with low pI
  • mitochondrial Smac protein
  • Second mitochondria-derived activator of caspase
  • SMAC
  • SMACmitochondrial


The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO. Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/caspase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SMAC/Diablo Antibody (NBP1-76772)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


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Bioinformatics Tool for SMAC/Diablo Antibody (NBP1-76772)

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Research Areas for SMAC/Diablo Antibody (NBP1-76772)

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Blogs on SMAC/Diablo.

Apoptosis and Necroptosis Part II: Inhibitors of apoptosis proteins (IAPs); Key regulators of the balance between necroptosis, apoptosis and survival
In the first installment of this two-part blog post titled "Apoptosis and Necroptosis: Important factors to identify both types of programmed cell death", the mechanisms by which cell death occurs and ways to identify these pathways were...  Read full blog post.

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Gene Symbol DIABLO