Western Blot: SMAC/Diablo Antibody [NBP1-76772] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: SMAC/Diablo NBP1-76772 (1 ug/mL), 1h incubation at RT in 5% ...read more
Immunocytochemistry/ Immunofluorescence: SMAC/Diablo Antibody [NBP1-76772] - Immunofluorescence of Smac in Mouse Spleen cells with Smac antibody at 10 ug/mL.
Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NBP1-76772] - Immunohistochemical staining of mouse spleen tissue using Smac antibody at 2 ug/ml.
Western Blot: SMAC/Diablo Antibody [NBP1-76772] - Analysis of Smac in mouse heart tissue lysate in the absence (A) or presence (B) of blocking peptide and in rat heart tissue lysate with Smac antibody at 1 ug /ml.
Independent Antibodies: Western Blot: SMAC/Diablo Antibody [NBP1-76772] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: ...read more
Smac antibody can be used for detection of Smac by Western blot at 0.5 to 1 mg/ml. A 25 kDa band can be detected.
25 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Peptide affinity purified
Alternate Names for SMAC/Diablo Antibody
diablo, IAP-binding mitochondrial protein
Direct IAP-binding protein with low pI
mitochondrial Smac protein
Second mitochondria-derived activator of caspase
The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO. Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/caspase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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