SMAC/Diablo Antibody (RM271)

Western Blot: SMAC/Diablo Antibody (RM271) [NBP2-61586] - HeLa and MCF-7 cells lysates using Anti-Smac/Diablo Rabbit Monoclonal Antibody at a 1:1000 dilution.

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody (RM271) [NBP2-61586] - Immunohistochemical staining of formalin fixed and paraffin embedded Human breast cancer tissue sections using NBP2-61586.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: RM271
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

SMAC/Diablo Antibody (RM271) Summary

• Description: Novus Biologicals Rabbit SMAC/Diablo Antibody (RM271) (NBP2-61586) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: A peptide corresponding to the C-terminus of Human SMAC/Diablo
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DIABLO
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol/PBS, 1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for SMAC/Diablo Antibody (RM271)

• 0610041G12Rik
• Diablo
• diablo, IAP-binding mitochondrial protein
• Direct IAP-binding protein with low pI
• mitochondrial Smac protein
• Second mitochondria-derived activator of caspase
• SMAC
• SMACmitochondrial

Background

Smac/DIABLO is a mitochondrial protein which activates various forms of apoptosis. Specifically, Smac exits the mitochondria and enters the cytosol where it initiates apoptosis by neutralizing members of the IAP family of apoptosis inhibitory proteins. Mitochondrial-mediated apoptosis is important in animal development and tissue homeostasis, with alterations resulting in a range of malignant disorders. Upon apoptotic stimuli, the mitochondrial proteins cytochrome c and Smac/DIABLO are released into the cytosol. The release of these proteins, however, occurs via different mechanisms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DIABLO