Slug Antibody (666633) [Unconjugated]

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Slug Monoclonal Antibody (Catalog # MAB7408, filled histogram) or isotype control antibody (MAB003, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

PC‑3 human prostate cancer cells were stained with Mouse Anti-Human Slug Monoclonal Antibody (Catalog # MAB7408, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: CyTOF-ready, Flow
• Clone: 666633
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Slug Antibody (666633) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Slug
  Met1-His268
  Accession # O43623
• Specificity: Detects human Slug in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Slug Antibody (666633) [Unconjugated]

• MGC10182
• Neural crest transcription factor Slug
• Protein snail homolog 2
• slug homolog, zinc finger protein (chicken)
• Slug
• SLUGH
• SLUGH1
• SLUGzinc finger protein
• SNAI2
• snail 2
• snail homolog 2 (Drosophila)
• SNAIL2
• WS2D
• zinc finger protein SNAI2

Background

SLUG, also known as SNAI2, is a zinc-finger transcription factor. SLUG is a transcriptional repressor which may suppress E-cadherin transcription and be involved in an epithelial to mesenchymal transition (EMT).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O43623(Human)