SLPI Antibody - BSA Free

Immunohistochemistry-Paraffin: SLPI Antibody [NBP1-89139] - Analysis in human cervix, uterine and skeletal muscle tissues. Corresponding SLPI RNA-seq data are presented for the same tissues.

Western Blot: SLPI Antibody [NBP1-89139] - Analysis in human cell line EFO-21.

Immunohistochemistry-Paraffin: SLPI Antibody [NBP1-89139] - Staining of human kidney shows no positivity in cells in tubules as expected.

Western Blot: SLPI Antibody [NBP1-89139] - Analysis in control (vector only transfected HEK293T lysate) and SLPI over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: SLPI Antibody [NBP1-89139] - Staining of human cervix, uterine shows high expression.

Immunohistochemistry-Paraffin: SLPI Antibody [NBP1-89139] - Staining of human lymph node shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: SLPI Antibody [NBP1-89139] - Staining of human skeletal muscle shows no positivity in myocytes.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SLPI Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SGKSFKAGVCPPKKSAQCLRYKKPECQSDWQCPGKKRCCPDTCGIKCLDPVDTPNPTRRKPGKCPVTYGQCLMLNPPNFCEMDGQCKRDLKCCMGMCGKSCV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLPI
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1:100 - 1:250
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  SLPI Protein (NBP1-89139PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SLPI Antibody - BSA Free

• ALK1
• ALPProtease inhibitor WAP4
• antileukoproteinase
• BLPISeminal proteinase inhibitor
• HUSI
• HUSI-I
• Mucus proteinase inhibitor
• secretory leukocyte peptidase inhibitor
• secretory leukocyte protease inhibitor (antileukoproteinase)
• Secretory leukocyte protease inhibitor
• SLPI
• WAP four-disulfide core domain protein 4
• WAP4HUSI-1
• WFDC4MPI

Background

SLPI encodes a secreted inhibitor which protects epithelial tissues from serine proteases. It is found in various secretions including seminal plasma, cervical mucus, and bronchial secretions, and has affinity for trypsin, leukocyte elastase, and cathepsin G. Its inhibitory effect contributes to the immune response by protecting epithelial surfaces from attack by endogenous proteolytic enzymes; the protein is also thought to have broad-spectrum anti-biotic activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLPI Antibody (NBP1-89139)(1)

Publication using NBP1-89139

• Rostami MR, LeBlanc MG, Strulovici-Barel Y et al. Smoking shifts human small airway epithelium club cells toward a lesser differentiated population NPJ genomic medicine 2021-09-08 [PMID: 34497273] (IHC-P, Human)

Bioinformatics

• Gene Symbol: SLPI