SLPI Antibody - BSA Free

Western Blot: SLPI Antibody [NBP1-76803] - Daudi cell lysate with SLPI antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry-Paraffin: SLPI Antibody [NBP1-76803] - Mouse lung tissue with SLPI antibody at 10 ug/ml.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SLPI Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SLPI Antibody - BSA Free (NBP1-76803) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-SLPI Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide from near the center of human SLPI. The immunogen is located within amino acids 60 - 110 of SLPI. Amino Acid Squence: CLDPVDTPNPTRRKPGK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLPI
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Use in ICC/IF reported in scientific literature (PMID: 28597116).
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  SLPI Peptide (NBP1-76803PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for SLPI Antibody - BSA Free

• ALK1
• ALPProtease inhibitor WAP4
• antileukoproteinase
• BLPISeminal proteinase inhibitor
• HUSI
• HUSI-I
• Mucus proteinase inhibitor
• secretory leukocyte peptidase inhibitor
• secretory leukocyte protease inhibitor (antileukoproteinase)
• Secretory leukocyte protease inhibitor
• SLPI
• WAP four-disulfide core domain protein 4
• WAP4HUSI-1
• WFDC4MPI

Background

Secretory leukocyte protease inhibitor (SLPI) is produced at mucosal surfaces, primarily the upper respiratory tract and is thought to play an important role in the antiprotease defense mechanism of the lung. SLPI forms inhibitory complexes with numerous proteolytic enzymes such as neutrophil elastase, and has been shown to possess anti-inflammatory, anti-viral, and antibacterial activities. Its expression in oral epithelial cells is stimulated by HIV-1 gp120, suggesting that SLPI is a component of the oral mucosal response to HIV-1. In peripheral blood monocytes, SLPI can inhibit NF-kappaB activation by inhibiting IkappaB degradation in the cytoplasm and competing for NF-kappaB binding sites in the nucleus. This attenuation of the inflammatory response may also act to suppress liver metastases and other cancer cell invasions, but promote blood-borne metastasis via an invasion-independent pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLPI Antibody (NBP1-76803)(5)

Publications using NBP1-76803

• Anne L. Rosen, Michael A. Lint, Dayne H. Voelker, Nicole M. Gilbert, Christopher P. Tomera, Jesús Santiago-Borges, Meghan A. Wallace, Thomas J. Hannan, Carey-Ann D. Burnham, Scott J. Hultgren, Andrew L. Kau, Matthew R. Chapman Secretory leukocyte protease inhibitor protects against severe urinary tract infection in mice mBio 2024-02-01 [PMID: 38270443]
• Rosen AL, Lint MA, Voelker DH et al. Secretory Leukocyte Protease Inhibitor Protects Against Severe Urinary Tract Infection in Mice bioRxiv : the preprint server for biology 2023-10-13 [PMID: 37873489] (IF/ICC, Mouse)
• Liu C, Gong J, Zhang Q et al. Dietary iron modulates gut microbiota and induces SLPI secretion to promote colorectal tumorigenesis Gut microbes 2023-06-14 [PMID: 37312410] (WB, IHC-P, Mouse)
• A Lebeau, D Bruyere, P Roncarati, P Peixoto, E Hervouet, G Cobraivill, B Taminiau, M Masson, C Gallego, G Mazzucchel, N Smargiasso, M Fleron, D Baiwir, E Hendrick, C Pilard, T Lerho, C Reynders, M Ancion, R Greimers, JC Twizere, G Daube, G Schlecht-L, F Bachelerie, JD Combes, P Melin, M Fillet, P Delvenne, P Hubert, M Herfs HPV infection alters vaginal microbiome through down-regulating host mucosal innate peptides used by Lactobacilli as amino acid sources Nature Communications, 2022-02-28;13(1):1076. 2022-02-28 [PMID: 35228537] (Immunocytochemistry/ Immunofluorescence, Human)
• Svensson D, Aidoukovitch A, Anders E et al. Secretory leukocyte protease inhibitor regulates human periodontal ligament cell production of pro-inflammatory cytokines Inflamm. Res. 2017-09-01 [PMID: 28597116] (ICC/IF, Human)

FAQs for SLPI Antibody (NBP1-76803). (Showing 1 - 1 of 1 FAQs).

1. What is the molecular weight of SLPI?
   • Here is information about this protein, UniProt P03973. Its theoretical MW is approximately 14 kDa. In our western blot testing with this antibody, we picked up the band a little bit higher than this, which is could be due to post translational modification.

Bioinformatics

• Gene Symbol: SLPI
• Uniprot:
  • CAA28187(Human)