SLPI Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

SLPI Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human SLPI
• Specificity: Detects human SLPI in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SLPI
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SLPI Antibody [Biotin]

• ALK1
• ALPProtease inhibitor WAP4
• antileukoproteinase
• BLPISeminal proteinase inhibitor
• HUSI
• HUSI-I
• Mucus proteinase inhibitor
• secretory leukocyte peptidase inhibitor
• secretory leukocyte protease inhibitor (antileukoproteinase)
• Secretory leukocyte protease inhibitor
• SLPI
• WAP four-disulfide core domain protein 4
• WAP4HUSI-1
• WFDC4MPI

Background

SLPI is a member of the Serpin family and is primarily expressed in secretory glandular epithelial cells. It is found in various secretory fluids and functions as a potent inhibitor of many proteolytic enzymes. SLPI can modulate cell proliferation and has been shown to have anti-inflammatory, anti-viral, and anti-bacterial activities (1, 2).

1. Zhang, D. et al. (2002) J. Biol. Chem. 277:29999.
2. Taggart, C.C. et al. (2002) J. Biol. Chem. 277:33648.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLPI Antibody (BAF1274)(6)

Publications using BAF1274

• S Nugteren, Y Simons-Oos, CL Menckeberg, DH Hulleman-v, DJ Lindenberg, JN Samsom Endogenous secretory leukocyte protease inhibitor (SLPI) inhibits microbial-induced monocyte activation European Journal of Immunology, 2022-12-22;0(0):. 2022-12-22 [PMID: 36480463] (ELISA Detection, IHC, Human)
• S Nugteren, SH den Uil, PM Delis-van, Y Simons-Oos, DJ Lindenberg, DH van Haafte, HBAC Stockmann, J Sanders, GA Meijer, RJA Fijneman, JN Samsom High expression of secretory leukocyte protease inhibitor (SLPI) in stage III micro-satellite stable colorectal cancer is associated with reduced disease recurrence Scientific Reports, 2022-07-16;12(1):12174. 2022-07-16 [PMID: 35842496] (IHC, Human)
• Sandrine Nugteren, Jeroen A.C.M. Goos, Pien M. Delis-van Diemen, Ytje Simons-Oosterhuis, Dicky J. Lindenbergh-Kortleve, Daniëlle H. van Haaften et al. Expression of the immune modulator secretory leukocyte protease inhibitor (SLPI) in colorectal cancer liver metastases and matched primary tumors is associated with a poorer prognosis OncoImmunology 10/26/2020 [PMID: 33101778]
• Jennifer Vandooren, Pieter Goeminne, Lise Boon, Estefania Ugarte-Berzal, Vasily Rybakin, Paul Proost et al. Neutrophils and Activated Macrophages Control Mucosal Immunity by Proteolytic Cleavage of Antileukoproteinase Frontiers in Immunology 5/28/2018 [PMID: 29892293]
• W Martin Kast Annexin A2 antibodies but not inhibitors of the annexin A2 heterotetramer impair productive HIV-1 infection of macrophages in vitro Virol. J., 2016-11-18;13(1):187. 2016-11-18 [PMID: 27863502] (ELISA Development (Detection), Human)
• Weldon S, McNally P, McElvaney NG, Elborn JS, McAuley DF, Wartelle J, Belaaouaj A, Levine RL, Taggart CC Decreased levels of secretory leucoprotease inhibitor in the pseudomonas-infected cystic fibrosis lung are due to neutrophil elastase degradation. J. Immunol., 2009-12-15;183(12):8148-56. 2009-12-15 [PMID: 20007580] (ELISA Development, Western Blot, Human)

Bioinformatics

• Gene Symbol: SLPI