SLPI Antibody [Unconjugated]

SLPI was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human SLPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1274) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

SLPI Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human SLPI
• Specificity: Detects human SLPI in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with mouse SLPI is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SLPI
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 2 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SLPI Antibody [Unconjugated]

• ALK1
• ALPProtease inhibitor WAP4
• antileukoproteinase
• BLPISeminal proteinase inhibitor
• HUSI
• HUSI-I
• Mucus proteinase inhibitor
• secretory leukocyte peptidase inhibitor
• secretory leukocyte protease inhibitor (antileukoproteinase)
• Secretory leukocyte protease inhibitor
• SLPI
• WAP four-disulfide core domain protein 4
• WAP4HUSI-1
• WFDC4MPI

Background

SLPI is a member of the Serpin family and is primarily expressed in secretory glandular epithelial cells. It is found in various secretory fluids and functions as a potent inhibitor of many proteolytic enzymes. SLPI can modulate cell proliferation and has been shown to have anti-inflammatory, anti-viral, and anti-bacterial activities (1, 2).

1. Zhang, D. et al. (2002) J. Biol. Chem. 277:29999.
2. Taggart, C.C. et al. (2002) J. Biol. Chem. 277:33648.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLPI Antibody (AF1274)(17)

Publications using AF1274

• Yu, Q;Tang, X;Hart, T;Homer, R;Belperron, AA;Bockenstedt, LK;Ring, A;Nakamura, A;Fikrig, E; Secretory leukocyte protease inhibitor influences periarticular joint inflammation in B. burgdorferi -infected mice bioRxiv : the preprint server for biology 2024-11-25 [PMID: 39651186] (Flow Cytometry, Immunocytochemistry, ELISA Detection, Bacteria - Borrelia burgdorferi)
• OF McElvaney, T Asakura, SL Meinig, JL Torres-Cas, RS Hagan, C Gabillard, MP Murphy, LB Thorne, A Borczuk, EP Reeves, RE Zumwalt, Y Mikami, TP Carroll, K Okuda, G Hogan, OJ McElvaney, J Clarke, NL McEvoy, PW Mallon, C McCarthy, G Curley, MC Wolfgang, RC Boucher, NG McElvaney Protease-anti-protease compartmentalization in SARS-CoV-2 ARDS: Therapeutic implications EBioMedicine, 2022-02-22;77(0):103894. 2022-02-22 [PMID: 35217407] (Western Blot, Human)
• A Morimoto, J Kikuta, K Nishikawa, T Sudo, M Uenaka, M Furuya, T Hasegawa, K Hashimoto, H Tsukazaki, S Seno, A Nakamura, D Okuzaki, F Sugihara, A Ninomiya, T Yoshimura, R Takao-Kawa, H Matsuda, M Ishii SLPI is a critical mediator that controls PTH-induced bone formation Nature Communications, 2021-04-09;12(1):2136. 4/9/2021 [PMID: 33837198]
• K Molloy, SG Smith, G Cagney, ET Dillon, CM Greene, NG McElvaney Characterisation of the Major Extracellular Proteases of Stenotrophomoas maltophilia and Their Effects on Pulmonary Antiproteases Pathogens, 2019-06-28;8(3):. 2019-06-28 [PMID: 31261656] (Western Blot, Bacteria - Stenotrophomonas maltophilia)
• Jennifer Vandooren, Pieter Goeminne, Lise Boon, Estefania Ugarte-Berzal, Vasily Rybakin, Paul Proost et al. Neutrophils and Activated Macrophages Control Mucosal Immunity by Proteolytic Cleavage of Antileukoproteinase Frontiers in Immunology 2018-05-28 [PMID: 29892293]
• QH Nguyen, N Pervolarak, K Blake, D Ma, RT Davis, N James, AT Phung, E Willey, R Kumar, E Jabart, I Driver, J Rock, A Goga, SA Khan, DA Lawson, Z Werb, K Kessenbroc Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity Nat Commun, 2018-05-23;9(1):2028. 2018-05-23 [PMID: 29795293] (IHC, Human)
• X Wang, Y Jin, YX Li, Y Yang Secretory leukocyte peptidase inhibitor expression and apoptosis effect in oral leukoplakia and oral squamous cell carcinoma Oncol. Rep., 2018-02-07;0(0):. 2/7/2018 [PMID: 29436667]
• Svensson D, Aidoukovitch A, Anders E et al. Secretory leukocyte protease inhibitor regulates human periodontal ligament cell production of pro-inflammatory cytokines Inflamm. Res. 9/1/2017 [PMID: 28597116]
• Hannila S, Siddiq M, Carmel J, Hou J, Chaudhry N, Bradley P, Hilaire M, Richman E, Hart R, Filbin M Secretory leukocyte protease inhibitor reverses inhibition by CNS myelin, promotes regeneration in the optic nerve, and suppresses expression of the transforming growth factor-beta signaling protein Smad2. J Neurosci, 2013-03-20;33(12):5138-51. 2013-03-20 [PMID: 23516280] (ChIP, Western Blot, Rat)
• Wen J, Nikitakis NG, Chaisuparat R, Greenwell-Wild T, Gliozzi M, Jin W, Adli A, Moutsopoulos N, Wu T, Warburton G, Wahl SM Secretory leukocyte protease inhibitor (SLPI) expression and tumor invasion in oral squamous cell carcinoma. Am. J. Pathol., 2011-06-01;178(6):2866-78. 2011-06-01 [PMID: 21641406] (IHC-P, Human)
• R.E. Fransès, D.F. McWilliams, P.I. Mapp, D.A. Walsh Osteochondral angiogenesis and increased protease inhibitor expression in OA Osteoarthritis and Cartilage 2010-04-01 [PMID: 20060952]
• Edstrom AM, Malm J, Frohm B, Martellini JA, Giwercman A, Morgelin M, Cole AM, Sorensen OE The major bactericidal activity of human seminal plasma is zinc-dependent and derived from fragmentation of the semenogelins. J. Immunol., 2008-09-01;181(5):3413-21. 2008-09-01 [PMID: 18714013] (Western Blot, Human)
• Nicol GR, Han M, Kim J, Birse CE, Brand E, Nguyen A, Mesri M, FitzHugh W, Kaminker P, Moore PA, Ruben SM, He T Use of an immunoaffinity-mass spectrometry-based approach for the quantification of protein biomarkers from serum samples of lung cancer patients. Mol. Cell Proteomics, 2008-04-03;7(10):1974-82. 2008-04-03 [PMID: 18388126] (Mass Spectrometry, Human)
• Jacobsen LC, Sorensen OE, Cowland JB, Borregaard N, Theilgaard-Monch K The secretory leukocyte protease inhibitor (SLPI) and the secondary granule protein lactoferrin are synthesized in myelocytes, colocalize in subcellular fractions of neutrophils, and are coreleased by activated neutrophils. J. Leukoc. Biol., 2008-02-19;83(5):1155-64. 2008-02-19 [PMID: 18285402] (ICC, Western Blot, Human)
• Simpkins FA, Devoogdt NM, Rasool N, Tchabo NE, Alejandro EU, Kamrava MM, Kohn EC The alarm anti-protease, secretory leukocyte protease inhibitor, is a proliferation and survival factor for ovarian cancer cells. Carcinogenesis, 2007-10-04;29(3):466-72. 2007-10-04 [PMID: 17916899] (Western Blot, Human)
• Kluger HM, Kluger Y, Gilmore-Hebert M, DiVito K, Chang JT, Rodov S, Mironenko O, Kacinski BM, Perkins AS, Sapi E cDNA microarray analysis of invasive and tumorigenic phenotypes in a breast cancer model. Lab. Invest., 2004-03-01;84(3):320-31. 2004-03-01 [PMID: 14767486] (ICC, Human)
• Valore EV, Park CH, Igreti SL, Ganz T Antimicrobial components of vaginal fluid. Am. J. Obstet. Gynecol., 2002-09-01;187(3):561-8. 2002-09-01 [PMID: 12237628] (Western Blot, Human)

Bioinformatics

• Gene Symbol: SLPI