SLC5A12 Antibody - BSA Free

Immunohistochemistry-Paraffin: SLC5A12 Antibody [NBP2-49322] - Staining in human kidney and skin tissues.. Corresponding SLC5A12 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: SLC5A12 Antibody [NBP2-49322] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.

Immunohistochemistry-Paraffin: SLC5A12 Antibody [NBP2-49322] - Staining of human small intestine shows weak to moderate membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: SLC5A12 Antibody [NBP2-49322] - Staining of human kidney shows strong membranous positivity in cells in proximal tubules.

Immunohistochemistry-Paraffin: SLC5A12 Antibody [NBP2-49322] - Staining of human skin shows no positivity in keratinocytes as expected.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SLC5A12 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SLC5A12 Antibody - BSA Free (NBP2-49322) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-SLC5A12 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: QGSTHAGGFHNVLEQSTNGSRLHIFDFDVDPLRRH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC5A12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID: 31708446)
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  SLC5A12 Recombinant Protein Antigen (NBP2-49322PEP)

Reactivity Notes

Mouse, Rat (80%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SLC5A12 Antibody - BSA Free

• Electroneutral sodium monocarboxylate cotransporter
• Low-affinity sodium-lactate cotransporter
• MGC52019
• SMCT2DKFZp564G223
• sodium-coupled monocarboxylate transporter 2
• sodium-iodide related cotransporter
• solute carrier family 5 (sodium/glucose cotransporter), member 12
• Solute carrier family 5 member 12

Background

Normal blood lactate is maintained at about 1.5 mM, and little filtered lactate is excreted in urine. Reabsorption oflactate is mediated by the low-affinity Na(+)-coupled lactate transporter SLC5A12 in the initial part of the proximaltubule and by the high-affinity Na(+)-coupled lactate transporter SLC5A8 (MIM 608044) in the distal proximal tubule(Gopal et al., 2007 (PubMed 17692818)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLC5A12 Antibody (NBP2-49322)(1)

Publication using NBP2-49322

• Pucino V, Certo M, Bulusu V et al. Lactate Buildup at the Site of Chronic Inflammation Promotes Disease by Inducing CD4+ T Cell Metabolic Rewiring Cell Metab. 2019-10-29 [PMID: 31708446] (IHC-P, ICC/IF, Human)

Bioinformatics

• Gene Symbol: SLC5A12